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Epitranscriptomics of Ischemic Heart Disease—The IHD-EPITRAN Study Design and Objectives

1
Department of Pharmacology, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland
2
Heart and Lung Center, Helsinki University Hospital, 00029 Helsinki, Finland
3
Tampere Heart Hospital, Tampere University Hospital, 33520 Tampere, Finland
4
Folkhälsan Research Center, 00250 Helsinki, Finland
5
Graduate School of Informatics, Department of Health Informatics, Middle East Technical University, 06800 Ankara, Turkey
6
Heart Center, Turku University Hospital and Department of Surgery, University of Turku, 20521 Turku, Finland
7
Research Unit of Surgery, Anesthesiology and Critical Care, University of Oulu, 90014 Oulu, Finland
8
Clinical Biobank Tampere, Tampere University Hospital, 33520 Tampere, Finland
9
Helsinki Institute of Life Science (HiLIFE), Meilahti Clinical Proteomics Core Facility, Department of Biochemistry and Developmental Biology, Faculty of Medicine, University of Helsinki, 00290 Helsinki, Finland
10
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Department of Biomedical Proteomics, 61-704 Poznan, Poland
11
Chemistry Unit, Finnish Food Authority, 00790 Helsinki, Finland
12
Helsinki Biobank, Hospital District of Helsinki and Uusimaa, 00029 Helsinki, Finland
13
Department of Chemical and Biological Engineering, College of Engineering, Koç University, 34450 Istanbul, Turkey
14
School of Medicine, Koç University, 34450 Istanbul, Turkey
15
Institute of Chemistry, University of Tartu, 50411 Tartu, Estonia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Alessandro Fatica
Int. J. Mol. Sci. 2021, 22(12), 6630; https://doi.org/10.3390/ijms22126630
Received: 24 May 2021 / Revised: 10 June 2021 / Accepted: 15 June 2021 / Published: 21 June 2021
(This article belongs to the Special Issue Coronary Syndromes – Advances in Diagnostics and Therapy)
Epitranscriptomic modifications in RNA can dramatically alter the way our genetic code is deciphered. Cells utilize these modifications not only to maintain physiological processes, but also to respond to extracellular cues and various stressors. Most often, adenosine residues in RNA are targeted, and result in modifications including methylation and deamination. Such modified residues as N-6-methyl-adenosine (m6A) and inosine, respectively, have been associated with cardiovascular diseases, and contribute to disease pathologies. The Ischemic Heart Disease Epitranscriptomics and Biomarkers (IHD-EPITRAN) study aims to provide a more comprehensive understanding to their nature and role in cardiovascular pathology. The study hypothesis is that pathological features of IHD are mirrored in the blood epitranscriptome. The IHD-EPITRAN study focuses on m6A and A-to-I modifications of RNA. Patients are recruited from four cohorts: (I) patients with IHD and myocardial infarction undergoing urgent revascularization; (II) patients with stable IHD undergoing coronary artery bypass grafting; (III) controls without coronary obstructions undergoing valve replacement due to aortic stenosis and (IV) controls with healthy coronaries verified by computed tomography. The abundance and distribution of m6A and A-to-I modifications in blood RNA are charted by quantitative and qualitative methods. Selected other modified nucleosides as well as IHD candidate protein and metabolic biomarkers are measured for reference. The results of the IHD-EPITRAN study can be expected to enable identification of epitranscriptomic IHD biomarker candidates and potential drug targets. View Full-Text
Keywords: biomarkers; epitranscriptomics; ischemic heart disease; N6-methyladenosine; m6A; adenosine-to-inosine; A-to-I; RNA modifications biomarkers; epitranscriptomics; ischemic heart disease; N6-methyladenosine; m6A; adenosine-to-inosine; A-to-I; RNA modifications
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MDPI and ACS Style

Sikorski, V.; Karjalainen, P.; Blokhina, D.; Oksaharju, K.; Khan, J.; Katayama, S.; Rajala, H.; Suihko, S.; Tuohinen, S.; Teittinen, K.; Nummi, A.; Nykänen, A.; Eskin, A.; Stark, C.; Biancari, F.; Kiss, J.; Simpanen, J.; Ropponen, J.; Lemström, K.; Savinainen, K.; Lalowski, M.; Kaarne, M.; Jormalainen, M.; Elomaa, O.; Koivisto, P.; Raivio, P.; Bäckström, P.; Dahlbacka, S.; Syrjälä, S.; Vainikka, T.; Vähäsilta, T.; Tuncbag, N.; Karelson, M.; Mervaala, E.; Juvonen, T.; Laine, M.; Laurikka, J.; Vento, A.; Kankuri, E. Epitranscriptomics of Ischemic Heart Disease—The IHD-EPITRAN Study Design and Objectives. Int. J. Mol. Sci. 2021, 22, 6630. https://doi.org/10.3390/ijms22126630

AMA Style

Sikorski V, Karjalainen P, Blokhina D, Oksaharju K, Khan J, Katayama S, Rajala H, Suihko S, Tuohinen S, Teittinen K, Nummi A, Nykänen A, Eskin A, Stark C, Biancari F, Kiss J, Simpanen J, Ropponen J, Lemström K, Savinainen K, Lalowski M, Kaarne M, Jormalainen M, Elomaa O, Koivisto P, Raivio P, Bäckström P, Dahlbacka S, Syrjälä S, Vainikka T, Vähäsilta T, Tuncbag N, Karelson M, Mervaala E, Juvonen T, Laine M, Laurikka J, Vento A, Kankuri E. Epitranscriptomics of Ischemic Heart Disease—The IHD-EPITRAN Study Design and Objectives. International Journal of Molecular Sciences. 2021; 22(12):6630. https://doi.org/10.3390/ijms22126630

Chicago/Turabian Style

Sikorski, Vilbert, Pasi Karjalainen, Daria Blokhina, Kati Oksaharju, Jahangir Khan, Shintaro Katayama, Helena Rajala, Satu Suihko, Suvi Tuohinen, Kari Teittinen, Annu Nummi, Antti Nykänen, Arda Eskin, Christoffer Stark, Fausto Biancari, Jan Kiss, Jarmo Simpanen, Jussi Ropponen, Karl Lemström, Kimmo Savinainen, Maciej Lalowski, Markku Kaarne, Mikko Jormalainen, Outi Elomaa, Pertti Koivisto, Peter Raivio, Pia Bäckström, Sebastian Dahlbacka, Simo Syrjälä, Tiina Vainikka, Tommi Vähäsilta, Nurcan Tuncbag, Mati Karelson, Eero Mervaala, Tatu Juvonen, Mika Laine, Jari Laurikka, Antti Vento, and Esko Kankuri. 2021. "Epitranscriptomics of Ischemic Heart Disease—The IHD-EPITRAN Study Design and Objectives" International Journal of Molecular Sciences 22, no. 12: 6630. https://doi.org/10.3390/ijms22126630

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