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Article

The Role of Processed Aloe vera Gel in Intestinal Tight Junction: An In Vivo and In Vitro Study

1
College of Pharmacy, Gachon University, Incheon 21936, Korea
2
College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
3
Univera Co., Ltd., Seoul 04782, Korea
*
Authors to whom correspondence should be addressed.
Authors contributed equally to this work.
Academic Editors: Kati Juuti-Uusitalo and Keijo Viiri
Int. J. Mol. Sci. 2021, 22(12), 6515; https://doi.org/10.3390/ijms22126515
Received: 27 May 2021 / Accepted: 14 June 2021 / Published: 17 June 2021
(This article belongs to the Special Issue Novel Biorelevant Intestinal Epithelial In Vitro Models)
Leaky gut is a condition of increased paracellular permeability of the intestine due to compromised tight junction barriers. In recent years, this affliction has drawn the attention of scientists from different fields, as a myriad of studies prosecuted it to be the silent culprit of various immune diseases. Due to various controversies surrounding its culpability in the clinic, approaches to leaky gut are restricted in maintaining a healthy lifestyle, avoiding irritating factors, and practicing alternative medicine, including the consumption of supplements. In the current study, we investigate the tight junction-modulating effects of processed Aloe vera gel (PAG), comprising 5–400-kD polysaccharides as the main components. Our results show that oral treatment of 143 mg/kg PAG daily for 10 days improves the age-related leaky gut condition in old mice, by reducing their individual urinal lactulose/mannitol (L/M) ratio. In concordance with in vivo experiments, PAG treatment at dose 400 μg/mL accelerated the polarization process of Caco-2 monolayers. The underlying mechanism was attributed to enhancement in the expression of intestinal tight junction-associated scaffold protein zonula occludens (ZO)-1 at the translation level. This was induced by activation of the MAPK/ERK signaling pathway, which inhibits the translation repressor 4E-BP1. In conclusion, we propose that consuming PAG as a complementary food has the potential to benefit high-risk patients. View Full-Text
Keywords: leaky gut; Aloe vera gel; tight junction; ZO-1; MAPK/ERK; 4E-BP1 leaky gut; Aloe vera gel; tight junction; ZO-1; MAPK/ERK; 4E-BP1
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MDPI and ACS Style

Le Phan, T.H.; Park, S.Y.; Jung, H.J.; Kim, M.W.; Cho, E.; Shim, K.-S.; Shin, E.; Yoon, J.-H.; Maeng, H.-J.; Kang, J.-H.; Oh, S.H. The Role of Processed Aloe vera Gel in Intestinal Tight Junction: An In Vivo and In Vitro Study. Int. J. Mol. Sci. 2021, 22, 6515. https://doi.org/10.3390/ijms22126515

AMA Style

Le Phan TH, Park SY, Jung HJ, Kim MW, Cho E, Shim K-S, Shin E, Yoon J-H, Maeng H-J, Kang J-H, Oh SH. The Role of Processed Aloe vera Gel in Intestinal Tight Junction: An In Vivo and In Vitro Study. International Journal of Molecular Sciences. 2021; 22(12):6515. https://doi.org/10.3390/ijms22126515

Chicago/Turabian Style

Le Phan, Thu Han, Se Yong Park, Hyun Jin Jung, Min Woo Kim, Eunae Cho, Kyu-Suk Shim, Eunju Shin, Jin-Ha Yoon, Han-Joo Maeng, Ju-Hee Kang, and Seung Hyun Oh. 2021. "The Role of Processed Aloe vera Gel in Intestinal Tight Junction: An In Vivo and In Vitro Study" International Journal of Molecular Sciences 22, no. 12: 6515. https://doi.org/10.3390/ijms22126515

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