Next Article in Journal
Association between Predicted Effects of TP53 Missense Variants on Protein Conformation and Their Phenotypic Presentation as Li-Fraumeni Syndrome or Hereditary Breast Cancer
Previous Article in Journal
All Good Things Must End: Termination of Receptor Tyrosine Kinase Signal
 
 
Article

LncRSPH9-4 Facilitates Meningitic Escherichia coli-Caused Blood–Brain Barrier Disruption via miR-17-5p/MMP3 Axis

by 1,2, 1,2, 1,2, 1,2, 1,2, 1,2,3,4, 1,2,3,4 and 1,2,3,4,*
1
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
2
Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China
3
Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture of the People’s Republic of China, Wuhan 430070, China
4
International Research Center for Animal Disease, Ministry of Science and Technology of the People’s Republic of China, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
Academic Editor: Lester Drewes
Int. J. Mol. Sci. 2021, 22(12), 6343; https://doi.org/10.3390/ijms22126343
Received: 1 June 2021 / Revised: 9 June 2021 / Accepted: 11 June 2021 / Published: 14 June 2021
(This article belongs to the Section Molecular Genetics and Genomics)
Brain microvascular endothelial cells (BMECs) constitute the structural and functional basis for the blood–brain barrier (BBB) and play essential roles in bacterial meningitis. Although the BBB integrity regulation has been under extensive investigation, there is little knowledge regarding the roles of long non-coding RNAs (lncRNAs) in this event. The present study aimed to investigate the roles of one potential lncRNA, lncRSPH9-4, in meningitic E. coli infection of BMECs. LncRSPH9-4 was cytoplasm located and significantly up-regulated in meningitic E. coli-infected hBMECs. Electrical cell-substrate impedance sensing (ECIS) measurement and Western blot assay demonstrated lncRSPH9-4 overexpression in hBMECs mediated the BBB integrity disruption. By RNA-sequencing analysis, 639 mRNAs and 299 miRNAs were significantly differentiated in response to lncRSPH9-4 overexpression. We further found lncRSPH9-4 regulated the permeability in hBMECs by competitively sponging miR-17-5p, thereby increasing MMP3 expression, which targeted the intercellular tight junctions. Here we reported the infection-induced lncRSPH9-4 aggravated disruption of the tight junctions in hBMECs, probably through the miR-17-5p/MMP3 axis. This finding provides new insights into the function of lncRNAs in BBB integrity during meningitic E. coli infection and provides the novel nucleic acid targets for future treatment of bacterial meningitis. View Full-Text
Keywords: brain microvascular endothelial cells; lncRSPH9-4; miR-17-5p; MMP3; tight junctions brain microvascular endothelial cells; lncRSPH9-4; miR-17-5p; MMP3; tight junctions
Show Figures

Figure 1

MDPI and ACS Style

Xu, B.; Yang, R.; Fu, J.; Yang, B.; Chen, J.; Tan, C.; Chen, H.; Wang, X. LncRSPH9-4 Facilitates Meningitic Escherichia coli-Caused Blood–Brain Barrier Disruption via miR-17-5p/MMP3 Axis. Int. J. Mol. Sci. 2021, 22, 6343. https://doi.org/10.3390/ijms22126343

AMA Style

Xu B, Yang R, Fu J, Yang B, Chen J, Tan C, Chen H, Wang X. LncRSPH9-4 Facilitates Meningitic Escherichia coli-Caused Blood–Brain Barrier Disruption via miR-17-5p/MMP3 Axis. International Journal of Molecular Sciences. 2021; 22(12):6343. https://doi.org/10.3390/ijms22126343

Chicago/Turabian Style

Xu, Bojie, Ruicheng Yang, Jiyang Fu, Bo Yang, Jiaqi Chen, Chen Tan, Huanchun Chen, and Xiangru Wang. 2021. "LncRSPH9-4 Facilitates Meningitic Escherichia coli-Caused Blood–Brain Barrier Disruption via miR-17-5p/MMP3 Axis" International Journal of Molecular Sciences 22, no. 12: 6343. https://doi.org/10.3390/ijms22126343

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop