Transcriptome Profiling of m6A mRNA Modification in Bovine Mammary Epithelial Cells Treated with Escherichia coli
, Yiya Yin 1, Chaohao Wang 1, Xin Tang 1,2, Tongxing Song 4, Aizhen Guo 2,3, Yingyu Chen 2,3 and Changmin Hu 1,*Round 1
Reviewer 1 Report
Reviewer report
Manuscript: Transcriptome profiling of m6A mRNA modification in bovine mammary epithelial cells treated with Escherichia coli.
This manuscript is something news in genetic control and diagnosis of E.coli mastitis. This study firstly presented the map of 26 RNA m6A modification of mastitis treated with E. coli, providing a basis for future research.
In this article authors have found a significant differencies in selected parameters between experimental and control groups which can indicated on the m6A mRNA modification.
And finally This findings clearly profile the m6A methylation of E. coli-induced MAC-T cells, and identify many differentially methylated genes, which may contribute to the diagnosis and treatment of mastitis.
In part Material and methods: I am not expert in this methods.
This part should put after introduction as number 2.
Finnaly, This manuscript look like a very good.
Author Response
Point: This manuscript is something news in genetic control and diagnosis of E.coli mastitis. This study firstly presented the map of 26 RNA m6A modification of mastitis treated with E. coli, providing a basis for future research. In this article authors have found a significant differences in selected parameters between experimental and control groups which can indicated on the m6A mRNA modification. And finally, these findings clearly profile the m6A methylation of E. coli-induced MAC-T cells, and identify many differentially methylated genes, which may contribute to the diagnosis and treatment of mastitis. In part Material and methods: I am not expert in this method. This part should put after introduction as number 2. Finally, this manuscript look like a very good.
Response: Thanks for your suggestion. According to the format requirements of the IJMS, the Materials and methods part should be placed after the Results part, and we put it as the number 4.
Reviewer 2 Report
The paper submitted by Ting and colleagues aimed to evaluate the in vitro assay of the modification of m6A mRNA in E. coli involved in bovine mastitis.
The research is well conducted and I don't have any comment.
My only request is to revise the English language before publishing the paper.
Author Response
Point: The paper submitted by Ting and colleagues aimed to evaluate the in vitro assay of the modification of m6A mRNA in E. coli involved in bovine mastitis. The research is well conducted and I don't have any comment. My only request is to revise the English language before publishing the paper.
Response: Thanks for your suggestion. The English language were revised extensively by MDPI English editors, and the English Editing Certificate was provided.
Author Response File: 
Author Response.pdf
