Inhibition of Tunneling Nanotubes between Cancer Cell and the Endothelium Alters the Metastatic Phenotype
Abstract
:1. Introduction
2. Results
2.1. Cancer Cells Acquire an Invasive Phenotype by Interacting with the Endothelium
2.2. Mechanism of Nanoscale Bridge Formation and Involvement of Small GTPase–Exocyst Complex
2.3. Action of Pharmacological Inhibition in Intercellular Transfer
2.4. Inhibiting Actin Polymerization Pathway Leads to a Reversal of Phenotypic Transition
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Culture
4.3. Co-Culture
4.4. Matrix Preparation
4.5. Drug Treatment
4.6. MTT Assay
4.7. siRNA Transfection
4.8. Flow Cytometry
4.9. Fluorescence Microscopy
4.10. TNT Counting
4.11. Western Blotting
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Dash, C.; Saha, T.; Sengupta, S.; Jang, H.L. Inhibition of Tunneling Nanotubes between Cancer Cell and the Endothelium Alters the Metastatic Phenotype. Int. J. Mol. Sci. 2021, 22, 6161. https://doi.org/10.3390/ijms22116161
Dash C, Saha T, Sengupta S, Jang HL. Inhibition of Tunneling Nanotubes between Cancer Cell and the Endothelium Alters the Metastatic Phenotype. International Journal of Molecular Sciences. 2021; 22(11):6161. https://doi.org/10.3390/ijms22116161
Chicago/Turabian StyleDash, Chinmayee, Tanmoy Saha, Shiladitya Sengupta, and Hae Lin Jang. 2021. "Inhibition of Tunneling Nanotubes between Cancer Cell and the Endothelium Alters the Metastatic Phenotype" International Journal of Molecular Sciences 22, no. 11: 6161. https://doi.org/10.3390/ijms22116161