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Article

Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks

1
Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy
2
Laboratory of Clinical Proteomics, “Giovanni Paolo II” Hospital, 73100 ASL-Lecce, Italy
3
Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), Université de Lille, INSERM, U1192, F-59000 Lille, France
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Center for Advanced Studies and Technology (CAST), University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, Italy
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Department of Innovative Technologies in Medicine & Dentistry, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, Italy
*
Authors to whom correspondence should be addressed.
Academic Editor: Ana Martínez
Int. J. Mol. Sci. 2021, 22(10), 5245; https://doi.org/10.3390/ijms22105245
Received: 19 March 2021 / Revised: 7 May 2021 / Accepted: 12 May 2021 / Published: 15 May 2021
(This article belongs to the Special Issue Protein Kinases and Their Inhibitors in CNS Diseases)
Protein kinase C (PKC) activation induces cellular reprogramming and differentiation in various cell models. Although many effectors of PKC physiological actions have been elucidated, the molecular mechanisms regulating oligodendrocyte differentiation after PKC activation are still unclear. Here, we applied a liquid chromatography–mass spectrometry (LC–MS/MS) approach to provide a comprehensive analysis of the proteome expression changes in the MO3.13 oligodendroglial cell line after PKC activation. Our findings suggest that multiple networks that communicate and coordinate with each other may finally determine the fate of MO3.13 cells, thus identifying a modular and functional biological structure. In this work, we provide a detailed description of these networks and their participating components and interactions. Such assembly allows perturbing each module, thus describing its physiological significance in the differentiation program. We applied this approach by targeting the Rho-associated protein kinase (ROCK) in PKC-activated cells. Overall, our findings provide a resource for elucidating the PKC-mediated network modules that contribute to a more robust knowledge of the molecular dynamics leading to this cell fate transition. View Full-Text
Keywords: PKC; differentiation; oligodendrocytes; signaling; mass spectrometry; cytoskeleton; ROCK PKC; differentiation; oligodendrocytes; signaling; mass spectrometry; cytoskeleton; ROCK
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MDPI and ACS Style

Damato, M.; Cardon, T.; Wisztorski, M.; Fournier, I.; Pieragostino, D.; Cicalini, I.; Salzet, M.; Vergara, D.; Maffia, M. Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks. Int. J. Mol. Sci. 2021, 22, 5245. https://doi.org/10.3390/ijms22105245

AMA Style

Damato M, Cardon T, Wisztorski M, Fournier I, Pieragostino D, Cicalini I, Salzet M, Vergara D, Maffia M. Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks. International Journal of Molecular Sciences. 2021; 22(10):5245. https://doi.org/10.3390/ijms22105245

Chicago/Turabian Style

Damato, Marina, Tristan Cardon, Maxence Wisztorski, Isabelle Fournier, Damiana Pieragostino, Ilaria Cicalini, Michel Salzet, Daniele Vergara, and Michele Maffia. 2021. "Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks" International Journal of Molecular Sciences 22, no. 10: 5245. https://doi.org/10.3390/ijms22105245

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