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Article

Knockout of the OsNAC006 Transcription Factor Causes Drought and Heat Sensitivity in Rice

1
College of Life Sciences, Nankai University, Tianjin 300071, China
2
Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China
3
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College, Yangzhou University, Yangzhou 225009, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(7), 2288; https://doi.org/10.3390/ijms21072288
Received: 27 February 2020 / Revised: 15 March 2020 / Accepted: 23 March 2020 / Published: 26 March 2020
(This article belongs to the Section Molecular Plant Sciences)
Rice (Oryza sativa) responds to various abiotic stresses during growth. Plant-specific NAM, ATAF1/2, and CUC2 (NAC) transcription factors (TFs) play an important role in controlling numerous vital growth and developmental processes. To date, 170 NAC TFs have been reported in rice, but their roles remain largely unknown. Herein, we discovered that the TF OsNAC006 is constitutively expressed in rice, and regulated by H2O2, cold, heat, abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellin (GA), NaCl, and polyethylene glycol (PEG) 6000 treatments. Furthermore, knockout of OsNAC006 using the CRISPR-Cas9 system resulted in drought and heat sensitivity. RNA sequencing (RNA-seq) transcriptome analysis revealed that OsNAC006 regulates the expression of genes mainly involved in response to stimuli, oxidoreductase activity, cofactor binding, and membrane-related pathways. Our findings elucidate the important role of OsNAC006 in drought responses, and provide valuable information for genetic manipulation to enhance stress tolerance in future plant breeding programs. View Full-Text
Keywords: rice; NAC transcription factor; abiotic stresses; CRISPR-Cas9; transcriptome analysis rice; NAC transcription factor; abiotic stresses; CRISPR-Cas9; transcriptome analysis
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MDPI and ACS Style

Wang, B.; Zhong, Z.; Wang, X.; Han, X.; Yu, D.; Wang, C.; Song, W.; Zheng, X.; Chen, C.; Zhang, Y. Knockout of the OsNAC006 Transcription Factor Causes Drought and Heat Sensitivity in Rice. Int. J. Mol. Sci. 2020, 21, 2288. https://doi.org/10.3390/ijms21072288

AMA Style

Wang B, Zhong Z, Wang X, Han X, Yu D, Wang C, Song W, Zheng X, Chen C, Zhang Y. Knockout of the OsNAC006 Transcription Factor Causes Drought and Heat Sensitivity in Rice. International Journal of Molecular Sciences. 2020; 21(7):2288. https://doi.org/10.3390/ijms21072288

Chicago/Turabian Style

Wang, Bo, Zhaohui Zhong, Xia Wang, Xiangyan Han, Deshui Yu, Chunguo Wang, Wenqin Song, Xuelian Zheng, Chengbin Chen, and Yong Zhang. 2020. "Knockout of the OsNAC006 Transcription Factor Causes Drought and Heat Sensitivity in Rice" International Journal of Molecular Sciences 21, no. 7: 2288. https://doi.org/10.3390/ijms21072288

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