Vaccarin Regulates Diabetic Chronic Wound Healing through FOXP2/AGGF1 Pathways
Abstract
:1. Introduction
2. Results
2.1. Effects of Vaccarin on High Glucose-Induced Cell Viability
2.2. Effects of Vaccarin on High Glucose-Induced Cell Migration
2.3. Activation of FOXP2/AGGF1 Pathway in Response to Vaccarin Treatment
2.4. The Cell Migration Promoting Effects of VACCARIN Dependent on FOXP2
2.5. FOXP2/AGGF1 Mediated the Activation of PI3K/Akt and Erk1/2 Pathways Induced by Vaccarin
2.6. Effects of Vaccarin on Diabetic Wound Healing
2.7. Vaccarin Promoted Diabetic Chronic Wound Healing by Mediating FOXP2/AGGF1 Activation
3. Discussion
4. Materials and Methods
4.1. Reagents and Chemicals
4.2. Cell Culture and Treatments
4.3. Cell Proliferation Assay
4.4. In vitro Wound Healing Assay
4.5. Transwell Assay
4.6. Real-Time PCR Analysis
4.7. Western Blotting
4.8. siRNA Interference
4.9. Animal Model and Treatments
4.10. Pressure Ulcer Model
4.11. Pressure Ulcer formation Assessments
4.12. Mechanical Pressure Algesia
4.13. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Liu, Y.; Sun, J.; Ma, X.; Li, S.; Ai, M.; Xu, F.; Qiu, L. Vaccarin Regulates Diabetic Chronic Wound Healing through FOXP2/AGGF1 Pathways. Int. J. Mol. Sci. 2020, 21, 1966. https://doi.org/10.3390/ijms21061966
Liu Y, Sun J, Ma X, Li S, Ai M, Xu F, Qiu L. Vaccarin Regulates Diabetic Chronic Wound Healing through FOXP2/AGGF1 Pathways. International Journal of Molecular Sciences. 2020; 21(6):1966. https://doi.org/10.3390/ijms21061966
Chicago/Turabian StyleLiu, Yixiao, Jiangnan Sun, Xinyu Ma, Shuangshuang Li, Min Ai, Fei Xu, and Liying Qiu. 2020. "Vaccarin Regulates Diabetic Chronic Wound Healing through FOXP2/AGGF1 Pathways" International Journal of Molecular Sciences 21, no. 6: 1966. https://doi.org/10.3390/ijms21061966