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Volume 21
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10.3390/ijms21051595
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Article

Investigation of a Novel Salt Stress-Responsive Pathway Mediated by Arabidopsis DEAD-Box RNA Helicase Gene AtRH17 Using RNA-Seq Analysis

by
Hye-Yeon Seok
1,†,
Linh Vu Nguyen
2,†,
Doai Van Nguyen
2,
Sun-Young Lee
3 and
Yong-Hwan Moon
1,2,4,*
1
Institute of Systems Biology, Pusan National University, Busan 46241, Korea
2
Department of Integrated Biological Science, Pusan National University, Busan 46241, Korea
3
Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
4
Department of Molecular Biology, Pusan National University, Busan 46241, Korea
*
Author to whom correspondence should be addressed.
†
The authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(5), 1595; https://doi.org/10.3390/ijms21051595
Received: 5 February 2020 / Revised: 23 February 2020 / Accepted: 25 February 2020 / Published: 26 February 2020
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Previously, we reported that overexpression of AtRH17, an Arabidopsis DEAD-box RNA helicase gene, confers salt stress-tolerance via a pathway other than the well-known salt stress-responsive pathways. To decipher the salt stress-responsive pathway in AtRH17-overexpressing transgenic plants (OXs), we performed RNA-Sequencing and identified 397 differentially expressed genes between wild type (WT) and AtRH17 OXs. Among them, 286 genes were upregulated and 111 genes were downregulated in AtRH17 OXs relative to WT. Gene ontology annotation enrichment and KEGG pathway analysis showed that the 397 upregulated and downregulated genes are involved in various biological functions including secretion, signaling, detoxification, metabolic pathways, catabolic pathways, and biosynthesis of secondary metabolites as well as in stress responses. Genevestigator analysis of the upregulated genes showed that nine genes, namely, LEA4-5, GSTF6, DIN2/BGLU30, TSPO, GSTF7, LEA18, HAI1, ABR, and LTI30, were upregulated in Arabidopsis under salt, osmotic, and drought stress conditions. In particular, the expression levels of LEA4-5, TSPO, and ABR were higher in AtRH17 OXs than in WT under salt stress condition. Taken together, our results suggest that a high AtRH17 expression confers salt stress-tolerance through a novel salt stress-responsive pathway involving nine genes, other than the well-known ABA-dependent and ABA-independent pathways. View Full-Text
Keywords: Arabidopsis; AtRH17; DEAD-box RNA helicase; RNA-Sequencing; salt stress; stress-responsive pathway Arabidopsis; AtRH17; DEAD-box RNA helicase; RNA-Sequencing; salt stress; stress-responsive pathway
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

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MDPI and ACS Style

Seok, H.-Y.; Nguyen, L.V.; Nguyen, D.V.; Lee, S.-Y.; Moon, Y.-H. Investigation of a Novel Salt Stress-Responsive Pathway Mediated by Arabidopsis DEAD-Box RNA Helicase Gene AtRH17 Using RNA-Seq Analysis. Int. J. Mol. Sci. 2020, 21, 1595. https://doi.org/10.3390/ijms21051595

AMA Style

Seok H-Y, Nguyen LV, Nguyen DV, Lee S-Y, Moon Y-H. Investigation of a Novel Salt Stress-Responsive Pathway Mediated by Arabidopsis DEAD-Box RNA Helicase Gene AtRH17 Using RNA-Seq Analysis. International Journal of Molecular Sciences. 2020; 21(5):1595. https://doi.org/10.3390/ijms21051595

Chicago/Turabian Style

Seok, Hye-Yeon, Linh V. Nguyen, Doai V. Nguyen, Sun-Young Lee, and Yong-Hwan Moon. 2020. "Investigation of a Novel Salt Stress-Responsive Pathway Mediated by Arabidopsis DEAD-Box RNA Helicase Gene AtRH17 Using RNA-Seq Analysis" International Journal of Molecular Sciences 21, no. 5: 1595. https://doi.org/10.3390/ijms21051595

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