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Open AccessArticle

microRNAs in Ex Vivo Human Adipose Tissue Derived Mesenchymal Stromal Cells (ASC) Undergo Rapid Culture-Induced Changes in Expression, Including miR-378 which Promotes Adipogenesis

1
School of Biological Sciences, University of Auckland, 1150 Auckland, New Zealand
2
Maurice Wilkins Centre, University of Auckland, 1150 Auckland, New Zealand
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(4), 1492; https://doi.org/10.3390/ijms21041492
Received: 23 January 2020 / Revised: 12 February 2020 / Accepted: 18 February 2020 / Published: 21 February 2020
(This article belongs to the Collection Regulation by Non-Coding RNAs)
There is clinical interest in using human adipose tissue-derived mesenchymal stromal cells (ASC) to treat a range of inflammatory and regenerative conditions. Aspects of ASC biology, including their regenerative potential and paracrine effect, are likely to be modulated, in part, by microRNAs, small RNA molecules that are embedded as regulators of gene-expression in most biological pathways. However, the effect of standard isolation and expansion protocols on microRNA expression in ASC is not well explored. Here, by using an untouched and enriched population of primary human ASC, we demonstrate that there are rapid and significant changes in microRNA expression when ASC are subjected to standard isolation and expansion methods. Functional studies focusing on miR-378 indicate that these changes in expression may have an impact on phenotype and function. Specifically, we found that increased levels of miR-378 significantly promoted adipogenesis in late passage ASC. These results are informative to maximizing the potential of ASC for use in various clinical applications, and they have implications for targeting microRNAs as a therapeutic strategy for obesity or metabolic disease. View Full-Text
Keywords: human adipose-derived mesenchymal stem/stromal cells; adipose-derived stem cells; mesenchymal stem cells; microRNAs; paracrine effect; immunomagnetic bead sorting; stromal vascular fraction human adipose-derived mesenchymal stem/stromal cells; adipose-derived stem cells; mesenchymal stem cells; microRNAs; paracrine effect; immunomagnetic bead sorting; stromal vascular fraction
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MDPI and ACS Style

Iminitoff, M.; Damani, T.; Williams, E.; Brooks, A.E.S.; Feisst, V.; Sheppard, H.M. microRNAs in Ex Vivo Human Adipose Tissue Derived Mesenchymal Stromal Cells (ASC) Undergo Rapid Culture-Induced Changes in Expression, Including miR-378 which Promotes Adipogenesis. Int. J. Mol. Sci. 2020, 21, 1492. https://doi.org/10.3390/ijms21041492

AMA Style

Iminitoff M, Damani T, Williams E, Brooks AES, Feisst V, Sheppard HM. microRNAs in Ex Vivo Human Adipose Tissue Derived Mesenchymal Stromal Cells (ASC) Undergo Rapid Culture-Induced Changes in Expression, Including miR-378 which Promotes Adipogenesis. International Journal of Molecular Sciences. 2020; 21(4):1492. https://doi.org/10.3390/ijms21041492

Chicago/Turabian Style

Iminitoff, Megan; Damani, Tanvi; Williams, Eloise; Brooks, Anna E.S.; Feisst, Vaughan; Sheppard, Hilary M. 2020. "microRNAs in Ex Vivo Human Adipose Tissue Derived Mesenchymal Stromal Cells (ASC) Undergo Rapid Culture-Induced Changes in Expression, Including miR-378 which Promotes Adipogenesis" Int. J. Mol. Sci. 21, no. 4: 1492. https://doi.org/10.3390/ijms21041492

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