Biapenem as a Novel Insight into Drug Repositioning against Particulate Matter-Induced Lung Injury
Abstract
:1. Introduction
2. Results
2.1. Effects of BIPM on PM2.5-Induced Lung Damage
2.2. Effects of BIPM on PM2.5-Mediated Vascular Barrier Disruption
2.3. Effects of BIPM on PM2.5-Induced Pulmonary Inflammation
2.4. Effects of BIPM on PM2.5-Induced Signaling Pathways
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Animals and Husbandry
4.3. Primary Culture of Mouse Lung Microvascular Endothelial Cells (MLMVECs)
4.4. Lung Wet/Dry Weight Ratio
4.5. Hematoxylin and Eosin (H&E) Staining
4.6. ELISA of Phosphorylated p38 MAPK, MPO, NO, IL-1 β, and TNF-α
4.7. Protein Concentration and Cell Count in the BALF
4.8. Permeability Assays
4.9. Western Blot Analysis
4.10. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Lee, W.; Baek, M.-C.; Kim, K.-M.; Bae, J.-S. Biapenem as a Novel Insight into Drug Repositioning against Particulate Matter-Induced Lung Injury. Int. J. Mol. Sci. 2020, 21, 1462. https://doi.org/10.3390/ijms21041462
Lee W, Baek M-C, Kim K-M, Bae J-S. Biapenem as a Novel Insight into Drug Repositioning against Particulate Matter-Induced Lung Injury. International Journal of Molecular Sciences. 2020; 21(4):1462. https://doi.org/10.3390/ijms21041462
Chicago/Turabian StyleLee, Wonhwa, Moon-Chang Baek, Kyung-Min Kim, and Jong-Sup Bae. 2020. "Biapenem as a Novel Insight into Drug Repositioning against Particulate Matter-Induced Lung Injury" International Journal of Molecular Sciences 21, no. 4: 1462. https://doi.org/10.3390/ijms21041462