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Structural Changes and Astrocyte Response of the Lateral Geniculate Nucleus in a Ferret Model of Ocular Hypertension

1
Department of Ophthalmology, University of Tokyo School of Medicine, Tokyo 113-8655, Japan
2
Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, Kanazawa 920-1192, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(4), 1339; https://doi.org/10.3390/ijms21041339
Received: 27 November 2019 / Revised: 4 February 2020 / Accepted: 12 February 2020 / Published: 17 February 2020
(This article belongs to the Section Molecular Neurobiology)
We investigated structural changes and astrocyte responses of the lateral geniculate nucleus (LGN) in a ferret model of ocular hypertension (OH). In 10 ferrets, OH was induced via the injection of cultured conjunctival cells into the anterior chamber of the right eye; six normal ferrets were used as controls. Anterograde axonal tracing with cholera toxin B revealed that atrophic damage was evident in the LGN layers receiving projections from OH eyes. Immunohistochemical analysis with antibodies against NeuN, glial fibrillary acidic protein (GFAP), and Iba-1 was performed to specifically label neurons, astrocytes, and microglia in the LGN. Significantly decreased NeuN immunoreactivity and increased GFAP and Iba-1 immunoreactivities were observed in the LGN layers receiving projections from OH eyes. Interestingly, the changes in the immunoreactivities were significantly different among the LGN layers. The C layers showed more severe damage than the A and A1 layers. Secondary degenerative changes in the LGN were also observed, including neuronal damage and astrocyte reactions in each LGN layer. These results suggest that our ferret model of OH is valuable for investigating damages during the retina–brain transmission of the visual pathway in glaucoma. The vulnerability of the C layers was revealed for the first time. View Full-Text
Keywords: LGN; ferret; glaucoma LGN; ferret; glaucoma
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Fujishiro, T.; Honjo, M.; Kawasaki, H.; Asaoka, R.; Yamagishi, R.; Aihara, M. Structural Changes and Astrocyte Response of the Lateral Geniculate Nucleus in a Ferret Model of Ocular Hypertension. Int. J. Mol. Sci. 2020, 21, 1339.

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