In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects

Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain

Diet, Human Microbiota and Health Group, Institute of Health Research of the Principality of Asturias (ISPA), 33011 Oviedo, Spain

Functionality and Ecology of Beneficial Microorganisms, Institute of Health Research of the Principality of Asturias (ISPA), 33011 Oviedo, Spain

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Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, 43121 Parma, Italy

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Digestive Service, Central University Hospital of Asturias (HUCA), 33011 Oviedo, Asturias, Spain

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Endocrinology and Nutrition Service, Central University Hospital of Asturias (HUCA), 33011 Oviedo, Asturias, Spain

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Endocrinology, Nutrition, Diabetes and Obesity Group, Institute of Health Research of the Principality of Asturias (ISPA), 33011 Oviedo, Spain

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β-Food Sciences Co., Chita 478-0046, Japan

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Department of Food and Cosmetic Science, Tokyo University of Agriculture, Abashiri 099-2493, Japan

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2020, 21(3), 906; https://doi.org/10.3390/ijms21030906

Received: 20 December 2019 / Revised: 13 January 2020 / Accepted: 28 January 2020 / Published: 30 January 2020

(This article belongs to the Special Issue Molecular Mechanisms of Bioactive Nutrients Promoting Health through Gut Microbiota)

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Abstract

The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis. Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality. Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults. Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide. Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography). Additionally, the effect on the bifidobacterial species was assessed (ITS-sequencing). Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line. In general, 1-kestose was the compound showing the largest effects. The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated. Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products. View Full-Text

Keywords: in vitro model; microbiota; prebiotics; gas production; obesity; functionality; HT29; RTCA; SCFA; bifidobacterial-ITS

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MDPI and ACS Style

Nogacka, A.M.; Salazar, N.; Arboleya, S.; Ruas-Madiedo, P.; Mancabelli, L.; Suarez, A.; Martinez-Faedo, C.; Ventura, M.; Tochio, T.; Hirano, K.; Endo, A.; G. de los Reyes-Gavilán, C.; Gueimonde, M. In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects. Int. J. Mol. Sci. 2020, 21, 906. https://doi.org/10.3390/ijms21030906

AMA Style

Nogacka AM, Salazar N, Arboleya S, Ruas-Madiedo P, Mancabelli L, Suarez A, Martinez-Faedo C, Ventura M, Tochio T, Hirano K, Endo A, G. de los Reyes-Gavilán C, Gueimonde M. In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects. International Journal of Molecular Sciences. 2020; 21(3):906. https://doi.org/10.3390/ijms21030906

Chicago/Turabian Style

Nogacka, Alicja M., Nuria Salazar, Silvia Arboleya, Patricia Ruas-Madiedo, Leonardo Mancabelli, Adolfo Suarez, Ceferino Martinez-Faedo, Marco Ventura, Takumi Tochio, Katsuaki Hirano, Akihito Endo, Clara G. de los Reyes-Gavilán, and Miguel Gueimonde. 2020. "In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects" International Journal of Molecular Sciences 21, no. 3: 906. https://doi.org/10.3390/ijms21030906

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In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects

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