Expression of Bitter Taste Receptors in the Intestinal Cells of Non-Human Primates
Molecular Biology Section, Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Aichi 4848506, Japan
Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo 1568502, Japan
Structural Bioscience for Taste Molecular Recognition, Graduate School of Medicine, Kyoto University, Kyoto 6068507, Japan
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(3), 902; https://doi.org/10.3390/ijms21030902
Received: 20 December 2019 / Revised: 27 January 2020 / Accepted: 28 January 2020 / Published: 30 January 2020
(This article belongs to the Section Molecular Biology)
(1) Background: Recent studies have investigated the expression of taste-related genes in the organs of various animals, including humans; however, data for additional taxa are needed to facilitate comparative analyses within and among species. (2) Methods: We investigated the expression of taste-related genes in the intestines of rhesus macaques, the non-human primates most commonly used in experimental models. (3) Results: Based on RNAseq and qRT-PCR, genes encoding bitter taste receptors and the G-protein gustducin were expressed in the gut of rhesus macaques. RNAscope analysis showed that one of the bitter receptors, TAS2R38, was expressed in some cells in the small intestine, and immunohistochemical analysis revealed the presence of T2R38-positive cells in the villi of the intestines. (4) Conclusions: These results suggest that bitter receptors are expressed in the gut of rhesus macaques, supporting the use of macaques as a model for studies of human taste, including gut analyses.