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Open AccessArticle

Degradation of Human Serum Albumin by Infrared Free Electron Laser Enhanced by Inclusion of a Salen-Type Schiff Base Zn (II) Complex

1
Department of Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan
2
FEL-TUS, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
3
Departamento de Química-Física, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(3), 874; https://doi.org/10.3390/ijms21030874
Received: 25 December 2019 / Revised: 22 January 2020 / Accepted: 27 January 2020 / Published: 29 January 2020
A salen-type Schiff base Zn(II) complex included in human serum albumin (HSA) protein was examined by UV-Vis, circular dichroism (CD), and fluorescence (PL) spectra. The formation of the composite material was also estimated by a GOLD program of ligand–protein docking simulation. A composite cast film of HSA and Zn(II) complex was prepared, and the effects of the docking of the metal complex on the degradation of protein molecules by mid-infrared free electron laser (IR-FEL) were investigated. The optimum wavelengths of IR-FEL irradiation to be used were based on experimental FT-IR spectra and vibrational analysis. Using TD-DFT results with 6-31G(d,p) and B3LYP, the IR spectrum of Zn(II) complex could be reasonably assigned. The respective wavelengths were 1652 cm−1 (HSA amide I), 1537 cm−1 (HSA amide II), and 1622 cm−1 (Zn(II) complex C=N). Degradation of HSA based on FT-IR microscope (IRM) analysis and protein secondary structure analysis program (IR-SSE) revealed that the composite material was degraded more than pure HSA or Zn(II) complex; the inclusion of Zn(II) complex enhanced destabilization of folding of HSA. View Full-Text
Keywords: IR-FEL; human serum albumin; Schiff base; Zn(II) complex; TD-DFT; fluorescence IR-FEL; human serum albumin; Schiff base; Zn(II) complex; TD-DFT; fluorescence
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MDPI and ACS Style

Onami, Y.; Kawasaki, T.; Aizawa, H.; Haraguchi, T.; Akitsu, T.; Tsukiyama, K.; Palafox, M.A. Degradation of Human Serum Albumin by Infrared Free Electron Laser Enhanced by Inclusion of a Salen-Type Schiff Base Zn (II) Complex. Int. J. Mol. Sci. 2020, 21, 874. https://doi.org/10.3390/ijms21030874

AMA Style

Onami Y, Kawasaki T, Aizawa H, Haraguchi T, Akitsu T, Tsukiyama K, Palafox MA. Degradation of Human Serum Albumin by Infrared Free Electron Laser Enhanced by Inclusion of a Salen-Type Schiff Base Zn (II) Complex. International Journal of Molecular Sciences. 2020; 21(3):874. https://doi.org/10.3390/ijms21030874

Chicago/Turabian Style

Onami, Yuika; Kawasaki, Takayasu; Aizawa, Hiroki; Haraguchi, Tomoyuki; Akitsu, Takashiro; Tsukiyama, Koichi; Palafox, Mauricio A. 2020. "Degradation of Human Serum Albumin by Infrared Free Electron Laser Enhanced by Inclusion of a Salen-Type Schiff Base Zn (II) Complex" Int. J. Mol. Sci. 21, no. 3: 874. https://doi.org/10.3390/ijms21030874

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