Next Article in Journal
The Effect of Selected Dental Materials Used in Conservative Dentistry, Endodontics, Surgery, and Orthodontics as Well as during the Periodontal Treatment on the Redox Balance in the Oral Cavity
Next Article in Special Issue
Bioactive Betulin and PEG Based Polyanhydrides for Use in Drug Delivery Systems
Previous Article in Journal
A Review of the Evidence for and against a Role for Mast Cells in Cutaneous Scarring and Fibrosis
Article

A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach

1
Pharmacology and Pharmaceutical Care Laboratory, Faculty of Pharmacy, University of Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
2
Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
3
iMed.ULisboa– Research Institute for Medicines, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal
4
Vasco da Gama Research Group (CIVG), Vasco da Gama University School (EUVG), 3020-210 Coimbra, Portugal
5
Dentistry Area, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
6
Biophysics Institute, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
7
Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
8
Instituto de Biofísica e Engenharia Biomédica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
9
Faculty of Veterinary Medicine (ULHT)/IGC, 1749-024 Lisboa, Portugal
10
Centro de Estudos do Ambiente e do Mar (CESAM), Faculdade de Ciências, Campo Grande, Universidade de Lisboa, 1749-016 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(24), 9681; https://doi.org/10.3390/ijms21249681
Received: 22 November 2020 / Revised: 14 December 2020 / Accepted: 15 December 2020 / Published: 18 December 2020
(This article belongs to the Special Issue Therapeutic Polymers for Drug Delivery)
Breast cancer is one of the most frequently diagnosed malignancies and common causes of cancer death in women. Recent studies suggest that environmental exposures to certain chemicals, such as 7,12-Dimethylbenzanthracene (DMBA), a chemical present in tobacco, may increase the risk of developing breast cancer later in life. The first-line treatments for breast cancer (surgery, chemotherapy or a combination of both) are generally invasive and frequently associated with severe side effects and high comorbidity. Consequently, novel approaches are strongly required to find more natural-like experimental models that better reflect the tumors’ etiology, physiopathology and response to treatments, as well as to find more targeted, efficient and minimally invasive treatments. This study proposes the development and an in deep biological characterization of an experimental model using DMBA-tumor-induction in Sprague-Dawley female rats. Moreover, a photothermal therapy approach using a near-infrared laser coupled with gold nanoparticles was preliminarily assessed. The gold nanoparticles were functionalized with Epidermal Growth Factor, and their physicochemical properties and in vitro effects were characterized. DMBA proved to be a very good and selective inductor of breast cancer, with 100% incidence and inducing an average of 4.7 tumors per animal. Epigenetic analysis showed that tumors classified with worst prognosis were hypomethylated. The tumor-induced rats were then subjected to a preliminary treatment using functionalized gold nanoparticles and its activation by laser (650–900 nm). The treatment outcomes presented very promising alterations in terms of tumor histology, confirming the presence of necrosis in most of the cases. Although this study revealed encouraging results as a breast cancer therapy, it is important to define tumor eligibility and specific efficiency criteria to further assess its application in breast cancer treatment on other species. View Full-Text
Keywords: breast cancer; experimental model; DMBA; laser photothermal therapy; gold nanoparticles; epigenetic alterations breast cancer; experimental model; DMBA; laser photothermal therapy; gold nanoparticles; epigenetic alterations
Show Figures

Graphical abstract

MDPI and ACS Style

Costa, E.; Ferreira-Gonçalves, T.; Cardoso, M.; Coelho, J.M.P.; Gaspar, M.M.; Faísca, P.; Ascensão, L.; Cabrita, A.S.; Reis, C.P.; Figueiredo, I.V. A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach. Int. J. Mol. Sci. 2020, 21, 9681. https://doi.org/10.3390/ijms21249681

AMA Style

Costa E, Ferreira-Gonçalves T, Cardoso M, Coelho JMP, Gaspar MM, Faísca P, Ascensão L, Cabrita AS, Reis CP, Figueiredo IV. A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach. International Journal of Molecular Sciences. 2020; 21(24):9681. https://doi.org/10.3390/ijms21249681

Chicago/Turabian Style

Costa, Eduardo, Tânia Ferreira-Gonçalves, Miguel Cardoso, João M.P. Coelho, Maria M. Gaspar, Pedro Faísca, Lia Ascensão, António S. Cabrita, Catarina P. Reis, and Isabel V. Figueiredo 2020. "A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach" International Journal of Molecular Sciences 21, no. 24: 9681. https://doi.org/10.3390/ijms21249681

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop