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Open AccessArticle

Structural Characterization of Glycerol Kinase from the Thermophilic Fungus Chaetomium thermophilum

1
Malopolska Centre of Biotechnology, Jagiellonian University, 7a Gronostajowa St., 30-387 Kraków, Poland
2
International Institute of Molecular and Cell Biology in Warsaw, 4 Ksiecia Trojdena St., 02-109 Warszawa, Poland
3
Department of Biology, Institute of Molecular Biology & Biophysics, Eidgenössische Technische Hochschule Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland
*
Author to whom correspondence should be addressed.
Equal contribution.
Int. J. Mol. Sci. 2020, 21(24), 9570; https://doi.org/10.3390/ijms21249570
Received: 2 November 2020 / Revised: 5 December 2020 / Accepted: 14 December 2020 / Published: 16 December 2020
(This article belongs to the Section Molecular Biology)
Glycerol is an organic compound that can be utilized as an alternative source of carbon by various organisms. One of the ways to assimilate glycerol by the cell is the phosphorylative catabolic pathway in which its activation is catalyzed by glycerol kinase (GK) and glycerol-3-phosphate (G3P) is formed. To date, several GK crystal structures from bacteria, archaea, and unicellular eukaryotic parasites have been solved. Herein, we present a series of crystal structures of GK from Chaetomium thermophilum (CtGK) in apo and glycerol-bound forms. In addition, we show the feasibility of an ADP-dependent glucokinase (ADPGK)-coupled enzymatic assay to measure the CtGK activity. New structures described in our work provide structural insights into the GK catalyzed reaction in the filamentous fungus and set the foundation for understanding the glycerol metabolism in eukaryotes. View Full-Text
Keywords: glycerol; glycerol kinase; glycerol metabolism; crystal structure; glycerol-3-phosphate; ADP-dependent glucokinase; ADPGK glycerol; glycerol kinase; glycerol metabolism; crystal structure; glycerol-3-phosphate; ADP-dependent glucokinase; ADPGK
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MDPI and ACS Style

Wilk, P.; Kuśka, K.; Wątor, E.; Małecki, P.H.; Woś, K.; Tokarz, P.; Dubin, G.; Grudnik, P. Structural Characterization of Glycerol Kinase from the Thermophilic Fungus Chaetomium thermophilum. Int. J. Mol. Sci. 2020, 21, 9570. https://doi.org/10.3390/ijms21249570

AMA Style

Wilk P, Kuśka K, Wątor E, Małecki PH, Woś K, Tokarz P, Dubin G, Grudnik P. Structural Characterization of Glycerol Kinase from the Thermophilic Fungus Chaetomium thermophilum. International Journal of Molecular Sciences. 2020; 21(24):9570. https://doi.org/10.3390/ijms21249570

Chicago/Turabian Style

Wilk, Piotr; Kuśka, Katarzyna; Wątor, Elżbieta; Małecki, Piotr H.; Woś, Klaudia; Tokarz, Piotr; Dubin, Grzegorz; Grudnik, Przemysław. 2020. "Structural Characterization of Glycerol Kinase from the Thermophilic Fungus Chaetomium thermophilum" Int. J. Mol. Sci. 21, no. 24: 9570. https://doi.org/10.3390/ijms21249570

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