Effects of Sphingosine-1-Phosphate on Cell Viability, Differentiation, and Gene Expression of Adipocytes
Abstract
:1. Introduction
2. Results
2.1. Effect of S1P on Cell Viability of Pre- and Differentiated Adipocytes
2.2. Effect of S1P on Adipocyte Differentiation
2.3. Effect of S1P on Gene Expression in Pre- and Differentiated Adipocytes
2.4. Gene Ontology (GO) Analysis
2.5. Pathway Entichment Analysis
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture of Preadipocytes
4.3. Adipocyte Differentiation
4.4. Cell Viability Assay (MTT Assay)
4.5. Oil Red O Staining
4.6. Transcriptome Analysis
4.7. DEG Analysis
4.8. Gene Ontology (GO) and Pathway Enrichment Analyses
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Upregulated DEGs | Downregulated DEGs | ||||||
---|---|---|---|---|---|---|---|
Pre- SGBS | Diff- SGBS | Pre- 3T3-L1 | Diff- 3T3-L1 | Pre- SGBS | Diff- SGBS | Pre- 3T3-L1 | Diff- 3T3-L1 |
PIK3C2B | HAS1 | Gm17332 | Fstl1 | FGF20 | EMX2 | Tmsb15b2 | Stard9 |
CTPS2 | CIDEC | Ces2c | Gm8281 | OLFM3 | ACAN | Gm14092 | Krtap16-3 |
ZNF506 | TNFRSF19 | Olfr1477 | Mier2 | FCER1A | IFIT1 | Gm13298 | Folr1 |
UBE2D3 | ZNF141 | Sftpc | Gm2237 | MRAP | SETD3 | Wdr17 | Usp26 |
TRABD2A | GPRC5A | Vmn1r46 | Atg7 | PHYHIPL | IRAK1 | Slc25a31 | Mcoln2 |
OSBPL1A | KLHL13 | Tcp11l1 | Lacc1 | CEMIP | FYCO1 | Gm20823 | Gad2 |
CISH | THRSP | Pou2f1 | Depdc7 | KLHDC8B | DENND3 | Olfr1436 | Gm17482 |
BIRC3 | ADAMTS9 | Ssx9 | Adipoq | CDKAL1 | NEO1 | Vmn1r227 | Gm17428 |
SCRIB | CAT | Olfr810 | B3gnt9 | TMEM170B | ARID1A | Olfr1323 | Pygl |
CCDC54 | SYN2 | Gm14025 | Trim13 | FOXO1 | STPG2 | Olfr825 | Ubash3b |
OR1B1 | CLHC1 | Gm3127 | Slx4 | SHISA4 | PODN | Ccdc152 | Rnase9 |
PLSCR5 | LIPE | Olfr807 | Lrrc9 | DIRC3 | HPS1 | Iqcj | Fam19a1 |
SPATA5 | AGBL2 | Lctl | Eif6 | PDE6A | CDH22 | Afp | Chd7 |
SLC25A25 | HACD3 | Clec7a | Rcor2 | BRD9 | TTLL12 | Oas1a | Gm11111 |
OR11H12 | GK | Ifna11 | Esp36 | FRY | RASSF3 | Gm20738 | Vpreb2 |
LOC340074 | NAT2 | Atp8a1 | Arhgap33 | QRSL1 | MICAL2 | Gm10477 | Gm2745 |
NDUFA8 | PDE11A | Sema3d | Ensa | FDXR | RGS7 | Olfr809 | Col16a1 |
KSR1 | CST2 | 5330417C22Rik | Sh2b1 | C17orf64 | ATP11A | Gm5662 | Prlr |
API5L1 | CD207 | Olfr392 | Pcdhb21 | KIRREL3 | LCTL | Gm19668 | Gm4399 |
CCDC160 | SENP8 | Klra23 | Sema4g | LY6G6C | PITPNM2 | Hsfy2 | Gm4406 |
OR2F1 | PDE1B | Gm10037 | D430020J02Rik | MUC3A | ADRBK1 | Rhbdl3 | Arhgef26 |
LONRF1 | EPHA4 | Olfr133 | Saal1 | DEFB104A | MINK1 | Tmprss11c | Pnmal1 |
TRIM49D2 | BCO1 | 4930402K13Rik | Olfr1076 | C9orf50 | LTBP2 | Lrit2 | Defa3 |
POGZ | HPD | Pde9a | Sssca1 | TMEM129 | MAN2A2 | S100a14 | Defa17 |
TACC3 | LAIR1 | Vmn1r4 | Fbxw17 | LAGE3 | SCUBE3 | Gm5538 | Vmn2r110 |
PTK2 | SLC38A4 | Fpr-rs6 | Zadh2 | SLC2A7 | GSK3A | Mlana | Lrrtm3 |
STK32A | ADIPOQ | 3425401B19Rik | Abi3bp | KRTAP1–5 | MKL | Sult2a2 | LOC102642717 |
CCL20 | LIPA | Lama3 | Fam180a | RAB25 | RFESD | Olfr773 | Gm10436 |
GMPR | SMOX | Pdk2 | Mtor | FLOT1 | CCDC181 | Car3 | Olfr1258 |
C17orf47 | NME8 | Catsperd | Foxm1 | MYO1E | RGS21 | Elmod1 | Defb25 |
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Wu, X.; Sakharkar, M.K.; Wabitsch, M.; Yang, J. Effects of Sphingosine-1-Phosphate on Cell Viability, Differentiation, and Gene Expression of Adipocytes. Int. J. Mol. Sci. 2020, 21, 9284. https://doi.org/10.3390/ijms21239284
Wu X, Sakharkar MK, Wabitsch M, Yang J. Effects of Sphingosine-1-Phosphate on Cell Viability, Differentiation, and Gene Expression of Adipocytes. International Journal of Molecular Sciences. 2020; 21(23):9284. https://doi.org/10.3390/ijms21239284
Chicago/Turabian StyleWu, Xiyuan, Meena Kishore Sakharkar, Martin Wabitsch, and Jian Yang. 2020. "Effects of Sphingosine-1-Phosphate on Cell Viability, Differentiation, and Gene Expression of Adipocytes" International Journal of Molecular Sciences 21, no. 23: 9284. https://doi.org/10.3390/ijms21239284