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Open AccessArticle

Rbm38 Reduces the Transcription Elongation Defect of the SMEK2 Gene Caused by Splicing Deficiency

1
Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan
2
Division of Gene Expression Mechanism, Institute for Comprehensive Medical Science, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan
3
Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8799; https://doi.org/10.3390/ijms21228799
Received: 15 October 2020 / Revised: 14 November 2020 / Accepted: 19 November 2020 / Published: 20 November 2020
Pre-mRNA splicing is an essential mechanism for ensuring integrity of the transcriptome in eukaryotes. Therefore, splicing deficiency might cause a decrease in functional proteins and the production of nonfunctional, aberrant proteins. To prevent the production of such aberrant proteins, eukaryotic cells have several mRNA quality control mechanisms. In addition to the known mechanisms, we previously found that transcription elongation is attenuated to prevent the accumulation of pre-mRNA under splicing-deficient conditions. However, the detailed molecular mechanism behind the defect in transcription elongation remains unknown. Here, we showed that the RNA binding protein Rbm38 reduced the transcription elongation defect of the SMEK2 gene caused by splicing deficiency. This reduction was shown to require the N- and C-terminal regions of Rbm38, along with an important role being played by the RNA-recognition motif of Rbm38. These findings advance our understanding of the molecular mechanism of the transcription elongation defect caused by splicing deficiency. View Full-Text
Keywords: pre-mRNA splicing; Rbm38; transcription elongation; spliceostatin A pre-mRNA splicing; Rbm38; transcription elongation; spliceostatin A
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MDPI and ACS Style

Muraoka, S.; Fukumura, K.; Hayashi, M.; Kataoka, N.; Mayeda, A.; Kaida, D. Rbm38 Reduces the Transcription Elongation Defect of the SMEK2 Gene Caused by Splicing Deficiency. Int. J. Mol. Sci. 2020, 21, 8799. https://doi.org/10.3390/ijms21228799

AMA Style

Muraoka S, Fukumura K, Hayashi M, Kataoka N, Mayeda A, Kaida D. Rbm38 Reduces the Transcription Elongation Defect of the SMEK2 Gene Caused by Splicing Deficiency. International Journal of Molecular Sciences. 2020; 21(22):8799. https://doi.org/10.3390/ijms21228799

Chicago/Turabian Style

Muraoka, Shintaro; Fukumura, Kazuhiro; Hayashi, Megumi; Kataoka, Naoyuki; Mayeda, Akila; Kaida, Daisuke. 2020. "Rbm38 Reduces the Transcription Elongation Defect of the SMEK2 Gene Caused by Splicing Deficiency" Int. J. Mol. Sci. 21, no. 22: 8799. https://doi.org/10.3390/ijms21228799

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