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Article

Fast, Precise, and Reliable Multiplex Detection of Potato Viruses by Loop-Mediated Isothermal Amplification

1
Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany
2
Böhm-Nordkartoffel Agrarproduktion GmbH&Co. OHG, Brüggerfeld 44, 29574 Ebstorf, Germany
3
Twyman Research Management Ltd., P.O. Box 493, Scarborough YO11 9FJ, UK
4
Institute of Plant Biology and Biotechnology, University of Münster, Schlossplatz 8, 48143 Münster, Germany
*
Author to whom correspondence should be addressed.
Equal contribution to the work.
Int. J. Mol. Sci. 2020, 21(22), 8741; https://doi.org/10.3390/ijms21228741
Received: 30 October 2020 / Revised: 16 November 2020 / Accepted: 17 November 2020 / Published: 19 November 2020
Potato is an important staple food crop in both developed and developing countries. However, potato plants are susceptible to several economically important viruses that reduce yields by up to 50% and affect tuber quality. One of the major threats is corky ringspot, which is a tuber necrosis caused by tobacco rattle virus (TRV). The appearance of corky ringspot symptoms on tubers prior to commercialization results in ≈ 45% of the tubers being downgraded in quality and value, while ≈ 55% are declared unsaleable. To improve current disease management practices, we have developed simple diagnostic methods for the reliable detection of TRV without RNA purification, involving minimalized sample handling (mini), subsequent improved colorimetric loop-mediated isothermal amplification (LAMP), and final verification by lateral-flow dipstick (LFD) analysis. Having optimized the mini-LAMP-LFD approach for the sensitive and specific detection of TRV, we confirmed the reliability and robustness of this approach by the simultaneous detection of TRV and other harmful viruses in duplex LAMP reactions. Therefore, our new approach offers breeders, producers, and farmers an inexpensive and efficient new platform for disease management in potato breeding and cultivation. View Full-Text
Keywords: loop-mediated isothermal amplification; lateral-flow dipstick; tobacco rattle virus loop-mediated isothermal amplification; lateral-flow dipstick; tobacco rattle virus
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MDPI and ACS Style

Edgü, G.; Freund, L.J.; Hartje, S.; Tacke, E.; Hofferbert, H.-R.; Twyman, R.M.; Noll, G.A.; Muth, J.; Prüfer, D. Fast, Precise, and Reliable Multiplex Detection of Potato Viruses by Loop-Mediated Isothermal Amplification. Int. J. Mol. Sci. 2020, 21, 8741. https://doi.org/10.3390/ijms21228741

AMA Style

Edgü G, Freund LJ, Hartje S, Tacke E, Hofferbert H-R, Twyman RM, Noll GA, Muth J, Prüfer D. Fast, Precise, and Reliable Multiplex Detection of Potato Viruses by Loop-Mediated Isothermal Amplification. International Journal of Molecular Sciences. 2020; 21(22):8741. https://doi.org/10.3390/ijms21228741

Chicago/Turabian Style

Edgü, Güven, Lena J. Freund, Stefanie Hartje, Eckhard Tacke, Hans-Reinhard Hofferbert, Richard M. Twyman, Gundula A. Noll, Jost Muth, and Dirk Prüfer. 2020. "Fast, Precise, and Reliable Multiplex Detection of Potato Viruses by Loop-Mediated Isothermal Amplification" International Journal of Molecular Sciences 21, no. 22: 8741. https://doi.org/10.3390/ijms21228741

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