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Humic Substances Mitigate the Impact of Tritium on Luminous Marine Bacteria. Involvement of Reactive Oxygen Species
Open AccessArticle

Enzymatic Responses to Low-Intensity Radiation of Tritium

1
Department of Medical and Biological Physics, Krasnoyarsk State Medical Academy, 660022 Krasnoyarsk, Russia
2
Biophysics Department, Siberian Federal University, 660041 Krasnoyarsk, Russia
3
Institute of Biophysics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, Russia
4
Department of Chemistry, Moscow State University, 119991 Moscow, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8464; https://doi.org/10.3390/ijms21228464
Received: 11 October 2020 / Revised: 6 November 2020 / Accepted: 9 November 2020 / Published: 11 November 2020
(This article belongs to the Special Issue Molecular Mechanisms of Responses to Low-Intensity Exposures)
The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations ≤ 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (<20%). The CLM-FP samples did not show any increase in the rate of the photobiochemical proton transfer under the exposure to HTO. The responses of the enzyme systems were compared to the ‘hormetic’ response of luminous marine bacterial cells studied earlier. We conclude that (1) the oxidoreductase reaction contributes significantly to the activation of the coupled enzyme system and bacterial cells by tritium, and (2) an increase in the organization level of biological systems promotes the hormesis phenomenon. View Full-Text
Keywords: hormesis; low-dose radiation; tritium; enzymes; bacterial luciferase; oxidoreductase; fluorescent protein hormesis; low-dose radiation; tritium; enzymes; bacterial luciferase; oxidoreductase; fluorescent protein
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MDPI and ACS Style

Rozhko, T.V.; Nemtseva, E.V.; Gardt, M.V.; Raikov, A.V.; Lisitsa, A.E.; Badun, G.A.; Kudryasheva, N.S. Enzymatic Responses to Low-Intensity Radiation of Tritium. Int. J. Mol. Sci. 2020, 21, 8464. https://doi.org/10.3390/ijms21228464

AMA Style

Rozhko TV, Nemtseva EV, Gardt MV, Raikov AV, Lisitsa AE, Badun GA, Kudryasheva NS. Enzymatic Responses to Low-Intensity Radiation of Tritium. International Journal of Molecular Sciences. 2020; 21(22):8464. https://doi.org/10.3390/ijms21228464

Chicago/Turabian Style

Rozhko, Tatiana V.; Nemtseva, Elena V.; Gardt, Maria V.; Raikov, Alexander V.; Lisitsa, Albert E.; Badun, Gennadii A.; Kudryasheva, Nadezhda S. 2020. "Enzymatic Responses to Low-Intensity Radiation of Tritium" Int. J. Mol. Sci. 21, no. 22: 8464. https://doi.org/10.3390/ijms21228464

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