Enhanced Radiosensitization for Cancer Treatment with Gold Nanoparticles through Sonoporation
Abstract
:1. Background
2. Methods and Materials
2.1. Hepatocellular Carcinoma Cell Lines
2.2. AuNPs
2.3. AuMBs
2.4. In Vitro Sonoporation System Setup
2.5. Cavitation Measurements
2.6. Assessment of Intracellular AuNPs
2.7. Colony Formation Assay
2.8. γ-H2AX Immunofluorescence Microscopy
2.9. Western Blot Analysis
2.10. Targeted AuMBs with VEGFR2 Conjugation
2.11. Targeted AuMB-Enhanced Ultrasound Imaging in Mice
2.12. AuMB-Sonoporation-Mediated Radiosensitization
2.13. Statistical Analysis
3. Results
3.1. Characterization of AuNPs and AuMBs
3.2. Acoustic Cavitation Effect and Intracellular Localization of AuNPs
3.3. Clonogenic Assessment of In Vitro Radiosensitization by AuMB Sonoporation
3.4. γ-H2AX Immunofluorescence Staining
3.5. Western Blotting
3.6. VEGFR2-Targed AuMBs to Enhance In Vivo Delivery
3.7. In Vivo Assessment of Xenograft Tumor-Growth Delay
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Lu, S.-L.; Liu, W.-W.; Cheng, J.C.-H.; Lin, L.-C.; Wang, C.-R.C.; Li, P.-C. Enhanced Radiosensitization for Cancer Treatment with Gold Nanoparticles through Sonoporation. Int. J. Mol. Sci. 2020, 21, 8370. https://doi.org/10.3390/ijms21218370
Lu S-L, Liu W-W, Cheng JC-H, Lin L-C, Wang C-RC, Li P-C. Enhanced Radiosensitization for Cancer Treatment with Gold Nanoparticles through Sonoporation. International Journal of Molecular Sciences. 2020; 21(21):8370. https://doi.org/10.3390/ijms21218370
Chicago/Turabian StyleLu, Shao-Lun, Wei-Wen Liu, Jason Chia-Hsien Cheng, Lien-Chieh Lin, Churng-Ren Chris Wang, and Pai-Chi Li. 2020. "Enhanced Radiosensitization for Cancer Treatment with Gold Nanoparticles through Sonoporation" International Journal of Molecular Sciences 21, no. 21: 8370. https://doi.org/10.3390/ijms21218370