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Article

Characterization of PC12 Cell Subclones with Different Sensitivities to Programmed Thermal Stimulation

1
Division of Oral Physiology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan
2
Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan
3
Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan
4
Laboratory of Health Chemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan
5
Division for Globalization Initiative, Liaison Center for Innovative Dentistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 8356; https://doi.org/10.3390/ijms21218356
Received: 21 October 2020 / Revised: 3 November 2020 / Accepted: 5 November 2020 / Published: 7 November 2020
(This article belongs to the Special Issue Kinase Signal Transduction 2020)
Neuritogenesis is the process underling nervous system regeneration; however, optimal extracellular signals that can promote neuronal regenerative activities require further investigation. Previously, we developed a novel method for inducing neuronal differentiation in rat PC12 cells using temperature-controlled repeated thermal stimulation (TRTS) with a heating plate. Based on neurogenic sensitivity to TRTS, PC12 cells were classified as either hyper- or hyposensitive. In this study, we aimed to investigate the mechanism of hyposensitivity by establishing two PC12-derived subclones according to TRTS sensitivity during differentiation: PC12-P1F1, a hypersensitive subclone, and PC12-P1D10, a hyposensitive subclone. To characterize these subclones, cell size and neuritogenesis were evaluated in subclones treated with nerve growth factor (NGF), bone morphogenetic protein (BMP), or various TRTS. No significant differences in cell size were observed among the parental cells and subclones. BMP4- or TRTS-induced neuritogenesis was increased in PC12-P1F1 cells compared to that in the parental cells, while no neuritogenesis was observed in PC12-P1D10 cells. In contrast, NGF-induced neuritogenesis was observed in all three cell lines. Furthermore, a BMP inhibitor, LDN-193189, considerably inhibited TRTS-induced neuritogenesis. These results suggest that the BMP pathway might be required for TRTS-induced neuritogenesis, demonstrating the useful aspects of these novel subclones for TRTS research. View Full-Text
Keywords: bone morphogenetic protein (BMP); nerve growth factor (NGF); neuritogenesis; PC12 cells; thermal stimulation bone morphogenetic protein (BMP); nerve growth factor (NGF); neuritogenesis; PC12 cells; thermal stimulation
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MDPI and ACS Style

Kudo, T.-a.; Tominami, K.; Izumi, S.; Hayashi, Y.; Noguchi, T.; Matsuzawa, A.; Hong, G.; Nakai, J. Characterization of PC12 Cell Subclones with Different Sensitivities to Programmed Thermal Stimulation. Int. J. Mol. Sci. 2020, 21, 8356. https://doi.org/10.3390/ijms21218356

AMA Style

Kudo T-a, Tominami K, Izumi S, Hayashi Y, Noguchi T, Matsuzawa A, Hong G, Nakai J. Characterization of PC12 Cell Subclones with Different Sensitivities to Programmed Thermal Stimulation. International Journal of Molecular Sciences. 2020; 21(21):8356. https://doi.org/10.3390/ijms21218356

Chicago/Turabian Style

Kudo, Tada-aki, Kanako Tominami, Satoshi Izumi, Yohei Hayashi, Takuya Noguchi, Atsushi Matsuzawa, Guang Hong, and Junichi Nakai. 2020. "Characterization of PC12 Cell Subclones with Different Sensitivities to Programmed Thermal Stimulation" International Journal of Molecular Sciences 21, no. 21: 8356. https://doi.org/10.3390/ijms21218356

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