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Open AccessArticle

Anti-Cancer Effect of Cordycepin on FGF9-Induced Testicular Tumorigenesis

1
Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan
2
Department of Physiology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan
3
Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40400, Taiwan
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 8336; https://doi.org/10.3390/ijms21218336
Received: 15 September 2020 / Revised: 9 October 2020 / Accepted: 3 November 2020 / Published: 6 November 2020
(This article belongs to the Section Molecular Oncology)
Cordycepin, a bioactive constituent from the fungus Cordyceps sinensis, could inhibit cancer cell proliferation and promote cell death via induction of cell cycle arrest, apoptosis and autophagy. Our novel finding from microarray analysis of cordycepin-treated MA-10 mouse Leydig tumor cells is that cordycepin down-regulated the mRNA levels of FGF9, FGF18, FGFR2 and FGFR3 genes in MA-10 cells. Meanwhile, the IPA-MAP pathway prediction result showed that cordycepin inhibited MA-10 cell proliferation by suppressing FGFs/FGFRs pathways. The in vitro study further revealed that cordycepin decreased FGF9-induced MA-10 cell proliferation by inhibiting the expressions of p-ERK1/2, p-Rb and E2F1, and subsequently reducing the expressions of cyclins and CDKs. In addition, a mouse allograft model was performed by intratumoral injection of FGF9 and/or intraperitoneal injection of cordycepin to MA-10-tumor bearing C57BL/6J mice. Results showed that FGF9-induced tumor growth in cordycepin-treated mice was significantly smaller than that in a PBS-treated control group. Furthermore, cordycepin decreased FGF9-induced FGFR1-4 protein expressions in vitro and in vivo. In summary, cordycepin inhibited FGF9-induced testicular tumor growth by suppressing the ERK1/2, Rb/E2F1, cell cycle pathways, and the expressions of FGFR1-4 proteins, suggesting that cordycepin can be used as a novel anticancer drug for testicular cancers. View Full-Text
Keywords: cordycepin; FGF9; cell proliferation; cell cycle; MA-10 mouse Leydig tumor cells; testicular cancer cordycepin; FGF9; cell proliferation; cell cycle; MA-10 mouse Leydig tumor cells; testicular cancer
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MDPI and ACS Style

Chang, M.-M.; Hong, S.-Y.; Yang, S.-H.; Wu, C.-C.; Wang, C.-Y.; Huang, B.-M. Anti-Cancer Effect of Cordycepin on FGF9-Induced Testicular Tumorigenesis. Int. J. Mol. Sci. 2020, 21, 8336. https://doi.org/10.3390/ijms21218336

AMA Style

Chang M-M, Hong S-Y, Yang S-H, Wu C-C, Wang C-Y, Huang B-M. Anti-Cancer Effect of Cordycepin on FGF9-Induced Testicular Tumorigenesis. International Journal of Molecular Sciences. 2020; 21(21):8336. https://doi.org/10.3390/ijms21218336

Chicago/Turabian Style

Chang, Ming-Min; Hong, Siou-Ying; Yang, Shang-Hsun; Wu, Chia-Ching; Wang, Chia-Yih; Huang, Bu-Miin. 2020. "Anti-Cancer Effect of Cordycepin on FGF9-Induced Testicular Tumorigenesis" Int. J. Mol. Sci. 21, no. 21: 8336. https://doi.org/10.3390/ijms21218336

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