Aging Induces Profound Changes in sncRNA in Rat Sperm and These Changes Are Modified by Perinatal Exposure to Environmental Flame Retardant
Abstract
:1. Introduction
2. Results
2.1. Changes in the Profile of Small RNA Expression
2.2. Functional Analysis of Age-Dependent Changes in miRNA Expression
2.3. BDE-47 Exposure-Dependent Changes
2.4. Functional Analysis of Age-Dependent Changes in piRNA Expression
3. Discussion
3.1. sncRNA in Sperm
3.2. Justification of the Model
3.3. Age-Dependent Changes in sncRNA Fraction
3.4. BDE-47 Modifies Effect of Aging
3.5. Limitations and Future Directions
4. Materials and Methods
4.1. Animals and Treatment
4.2. Extraction of Sperm RNA
4.3. Preparation of sncRNA Libraries and Sequencing
4.4. Identification of Differentially Expressed Small RNA
4.5. Statistical Analysis
4.6. Functional Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Effect of Age | Effect of Exposure | |||
---|---|---|---|---|
Control | BDE-47-Exposed | PND65 | PND120 | |
miRNA * | 249 | 68 | 1 | 18 |
piRNA | 908 | 44 | 0 | 0 |
tRNA | 227 | 53 | 0 | 6 |
Targets | Identified Non-Age-Dependent piRNA | Identified Age-Dependent piRNA | Enrichment, Fold Change | p-Value | Direction of Change in Age-Dependent Genes |
---|---|---|---|---|---|
All | 36,551 | 980 | -- | -- | 139 down 841 up |
LINE | 1557 | 33 | −1.2 | 0.40 | 4 down 19 up |
SINE | 1648 | 47 | 1.1 | 0.33 | 6 down 41 up |
LTR | 521 | 80 | 5.3 | <0.00001 | 13 down 67 up |
Satellite | 52 | 1 | −1.28 | 1 | 1 up |
Protein-coding | 353 | 142 | 11.8 | <0.00001 | 24 down 118 up |
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Suvorov, A.; Pilsner, J.R.; Naumov, V.; Shtratnikova, V.; Zheludkevich, A.; Gerasimov, E.; Logacheva, M.; Sergeyev, O. Aging Induces Profound Changes in sncRNA in Rat Sperm and These Changes Are Modified by Perinatal Exposure to Environmental Flame Retardant. Int. J. Mol. Sci. 2020, 21, 8252. https://doi.org/10.3390/ijms21218252
Suvorov A, Pilsner JR, Naumov V, Shtratnikova V, Zheludkevich A, Gerasimov E, Logacheva M, Sergeyev O. Aging Induces Profound Changes in sncRNA in Rat Sperm and These Changes Are Modified by Perinatal Exposure to Environmental Flame Retardant. International Journal of Molecular Sciences. 2020; 21(21):8252. https://doi.org/10.3390/ijms21218252
Chicago/Turabian StyleSuvorov, Alexander, J. Richard Pilsner, Vladimir Naumov, Victoria Shtratnikova, Anna Zheludkevich, Evgeny Gerasimov, Maria Logacheva, and Oleg Sergeyev. 2020. "Aging Induces Profound Changes in sncRNA in Rat Sperm and These Changes Are Modified by Perinatal Exposure to Environmental Flame Retardant" International Journal of Molecular Sciences 21, no. 21: 8252. https://doi.org/10.3390/ijms21218252