Nonanal Stimulates Growth Factors via Cyclic Adenosine Monophosphate (cAMP) Signaling in Human Hair Follicle Dermal Papilla Cells
Abstract
:1. Introduction
2. Results
2.1. Nonanal Increases Cell Proliferation
2.2. Nonanal Enhances Gene Expression of Growth Factors
2.3. Nonanal Does Not Affect the β-Catenin Signaling Pathway
2.4. Nonanal Activates the cAMP/PKA Signaling Pathway
2.5. cAMP Is Involved in Nonanal-Induced DPC Proliferation
2.6. cAMP Is Implicated in Nonanal-Induced Gene Expression of Growth Factors
2.7. Nonanal Promotes Cell Migration via cAMP
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. WST-1 Assay
4.3. cAMP Measurements
4.4. Real-Time PCR
4.5. Western Blotting
4.6. Migration Assay
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene Name | Primer Sequences (5′→ 3′) |
---|---|
Catenin beta 1 (CTNNB1) | F: CCGACACCAAGAAGCAGAGATG R: GTGGGATGGTGGGTGTAAGAG |
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) | F: AAATCAAGTGGGGCGATGC R: AGGGGGCAGAGATGATGACC |
Insulin-like growth factor-1 (IGF-1) | F: TTGCTCTCAACATCTCCCATCT R: TGCATCTTCACCTTCAAGAAAT |
Keratinocyte growth factor (KGF) | F: AGAGAAGGGATGCTGGAGGT R: CGTAAGGGGCACTGTTTTAGAG |
Lymphoid enhancer factor 1 (LEF1) | F: CAACCCTACCCATCCTCACTG R: GGCTCCTGCTCCTTTCTCTGT |
Vascular endothelial growth factor (VEGF) | F: CTTCTGAGTTGCCCAGGAGA R: GGATGGAGGAAGGTCAACCA |
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Park, S.; Kang, W.; Choi, D.; Son, B.; Park, T. Nonanal Stimulates Growth Factors via Cyclic Adenosine Monophosphate (cAMP) Signaling in Human Hair Follicle Dermal Papilla Cells. Int. J. Mol. Sci. 2020, 21, 8054. https://doi.org/10.3390/ijms21218054
Park S, Kang W, Choi D, Son B, Park T. Nonanal Stimulates Growth Factors via Cyclic Adenosine Monophosphate (cAMP) Signaling in Human Hair Follicle Dermal Papilla Cells. International Journal of Molecular Sciences. 2020; 21(21):8054. https://doi.org/10.3390/ijms21218054
Chicago/Turabian StylePark, Soyoon, Wesuk Kang, Dabin Choi, Bomin Son, and Taesun Park. 2020. "Nonanal Stimulates Growth Factors via Cyclic Adenosine Monophosphate (cAMP) Signaling in Human Hair Follicle Dermal Papilla Cells" International Journal of Molecular Sciences 21, no. 21: 8054. https://doi.org/10.3390/ijms21218054