Co-Culture of Primary Human Coronary Artery and Internal Thoracic Artery Endothelial Cells Results in Mutually Beneficial Paracrine Interactions
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Co-Culture Model
4.2. RT-qPCR
4.3. Western Blotting
4.4. Secretome Profiling
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
CABG | Coronary artery bypass grafting |
SV | Saphenous vein |
ITA | Internal thoracic artery |
ECs | Endothelial cells |
HITAEC | Human internal thoracic artery endothelial cells |
eNOS | Endothelial nitric oxide synthase |
VEGF | Vascular endothelial growth factor |
HSaVEC | Human saphenous vein endothelial cells |
HCAEC | Human coronary artery endothelial cells |
EndoMT | Endothelial-to-mesenchymal transition |
RNA | Ribonucleic acid |
IL | Interleukin |
CXCL | Chemokine (C-X-C motif) ligand |
VCAM | Vascular cell adhesion molecule |
ICAM | Intercellular cell adhesion molecule |
SELE | E-selectin |
SNAI | Snail family transcriptional repressor |
TWIST | Twist family basic helix-loop-helix transcription factor |
ZEB | Zinc finger E-box binding homeobox |
CDH | Cadherin |
VE-cadherin | Vascular endothelial cadherin |
N-cadherin | Neural cadherin |
HEY | Hairy enhancer of split-related family basic helix-loop-helix transcription factor with YRPW motif |
HES | Hairy enhancer of split family helix-loop-helix transcription factor |
RT-qPCR | Reverse transcription-quantitative polymerase chain reaction |
CD | Cluster of differentiation |
LAD | Left anterior descending coronary artery |
PBS | Phosphate-buffered saline |
RIPA | Radioimmunoprecipitation assay |
cDNA | Complementary DNA |
PECAM | Platelet endothelial cell adhesion molecule |
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Gene | Forward Primer Sequence | Reverse Primer Sequence |
---|---|---|
NOS3 | 5′-GTGATGGCGAAGCGAGTGAAG-3′ | 5′-CCGAGCCCGAACACACAGAAC-3′ |
IL6 | 5′-GGCACTGGCAGAAAACAACC-3′ | 5′-GCAAGTCTCCTCATTGAATCC-3′ |
CXCL8 | 5′-CAGAGACAGCAGAGCACAC-3′ | 5′-AGTTCTTTAGCACTCCTTGGC-3′ |
VCAM1 | 5′-CGTCTTGGTCAGCCCTTCCT-3′ | 5′-ACATTCATATACTCCCGCATCCTTC-3′ |
ICAM1 | 5′-TTGGGCATAGAGACCCCGTT-3′ | 5′-GCACATTGCTCAGTTCATACACC-3′ |
SELE | 5′-GCACAGCCTTGTCCAACC-3′ | 5′-ACCTCACCAAACCCTTCG-3′ |
NAI1 | 5′-CAGACCCACTCAGATGTCAAGAA-3′ | 5′-GGGCAGGTATGGAGAGGAAGA-3′ |
SNAI2 | 5′-ACTCCGAAGCCAAATGACAA-3′ | 5′-CTCTCTCTGTGGGTGTGTGT-3′ |
TWIST1 | 5′-GTCCGCAGTCTTACGAGGAG-3′ | 5′-GCTTGAGGGTCTGAATCTTGCT-3′ |
ZEB1 | 5′-GATGATGAATGCGAGTCAGATGC-3′ | 5′-ACAGCAGTGTCTTGTTGTTGT-3′ |
CDH5 | 5′-AAGCGTGAGTCGCAAGAATG-3′ | 5′-TCTCCAGGTTTTCGCCAGTG-3′ |
CDH2 | 5′-GCTTCTGGTGAAATCGCATTA-3′ | 5′-AGTCTCTCTTCTGCCTTTGTAG-3′ |
PECAM1 | 5′-TGGCGCATGCCTGTAGTA-3′ | 5′-TCCGTTTCCTGGGTTCAA-3′ |
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Shishkova, D.; Markova, V.; Sinitsky, M.; Tsepokina, A.; Frolov, A.; Zagorodnikov, N.; Bogdanov, L.; Kutikhin, A. Co-Culture of Primary Human Coronary Artery and Internal Thoracic Artery Endothelial Cells Results in Mutually Beneficial Paracrine Interactions. Int. J. Mol. Sci. 2020, 21, 8032. https://doi.org/10.3390/ijms21218032
Shishkova D, Markova V, Sinitsky M, Tsepokina A, Frolov A, Zagorodnikov N, Bogdanov L, Kutikhin A. Co-Culture of Primary Human Coronary Artery and Internal Thoracic Artery Endothelial Cells Results in Mutually Beneficial Paracrine Interactions. International Journal of Molecular Sciences. 2020; 21(21):8032. https://doi.org/10.3390/ijms21218032
Chicago/Turabian StyleShishkova, Daria, Victoria Markova, Maxim Sinitsky, Anna Tsepokina, Alexey Frolov, Nikita Zagorodnikov, Leo Bogdanov, and Anton Kutikhin. 2020. "Co-Culture of Primary Human Coronary Artery and Internal Thoracic Artery Endothelial Cells Results in Mutually Beneficial Paracrine Interactions" International Journal of Molecular Sciences 21, no. 21: 8032. https://doi.org/10.3390/ijms21218032