Phospho-Mimetic Mutation at Ser602 Inactivates Human TRPA1 Channel
Abstract
:1. Introduction
2. Results
2.1. Phophomimetic Mutation S602D Abrogates Voltage-Dependent Activation of TRPA1
2.2. Salt-Bridge Formation is Not Involved in the Effects Caused by the S602D Mutation
2.3. Molecular Dynamics Simulations of the Phosphorylation of TRPA1 at Ser602
3. Discussion
4. Materials and Methods
4.1. Cell Culture, Constructs, and Transfection
4.2. Electrophysiology
4.3. Confocal Microscopy
4.4. Molecular Modeling
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
TRPA1 | Transient receptor potential ankyrin subtype 1 |
JT010 | 2-chloro-N-(4-(4-methoxyphenyl)thiazol-2-yl)-N-(3-methoxypropyl)-acetamide |
AGC | Kinase family named after the protein kinase A, G, and C families |
CMGC | Kinase family named after the initials of its subfamily members, including cyclin-dependent kinase, mitogen-activated protein kinase, glycogen synthase kinase and CDC-like kinase |
AR16 | Ankyrin repeat 16 |
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Predicted Protein Kinase | Prediction Server |
---|---|
Protein kinase A | GPS 5.0 [49], PhosphoPICK [50] |
Protein kinase C | GPS 5.0, NetPhos [51], NetPhorest, NetworKIN [52] |
Protein kinase D1 | GPS 5.0 [49], PhosphoPICK [50] |
Glycogen synthase kinase-3 | GPS 5.0 [49] |
AKT2 kinase | GPS 5.0 [49] |
Rho-associated protein kinase | PhoScan [53] |
Mitogen-activated protein (MAP) kinase 3 | Musite [54] |
MAP kinase-activated protein kinase 5 | GPS 5.0 [49] |
ULK1 protein kinase | GPS 5.0 [49] |
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Barvikova, K.; Barvik, I.; Sinica, V.; Zimova, L.; Vlachova, V. Phospho-Mimetic Mutation at Ser602 Inactivates Human TRPA1 Channel. Int. J. Mol. Sci. 2020, 21, 7995. https://doi.org/10.3390/ijms21217995
Barvikova K, Barvik I, Sinica V, Zimova L, Vlachova V. Phospho-Mimetic Mutation at Ser602 Inactivates Human TRPA1 Channel. International Journal of Molecular Sciences. 2020; 21(21):7995. https://doi.org/10.3390/ijms21217995
Chicago/Turabian StyleBarvikova, Kristyna, Ivan Barvik, Viktor Sinica, Lucie Zimova, and Viktorie Vlachova. 2020. "Phospho-Mimetic Mutation at Ser602 Inactivates Human TRPA1 Channel" International Journal of Molecular Sciences 21, no. 21: 7995. https://doi.org/10.3390/ijms21217995