Survival of Lung Cancer Patients Dependent on the LOH Status for DMP1, ARF, and p53
Abstract
:1. Introduction
2. Results
2.1. Impacts of LOH for hDMP1, ARF/INK4a, and p53 on NSCLC Survival
2.2. The Impact of hDMP1 LOH on NSCLC Survival with p53 LOH
2.3. Cyclin D1 Overexpression and YY1 Amplification and NSCLC Survival
3. Discussion
4. Materials and Methods
4.1. Human Lung Cancer Samples
4.2. Loss of Heterozygosity (LOH) and Sequencing Analyses of Human Lung Cancer Specimen
4.3. Statistical Analyses for Mutual Exclusiveness of LOH
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Patient ID | hDMP1 | INK4a/ARF | INK4a/ARF | I/A LOH ME | p53 | p53 LOH ME | p53 IHC | p53 IHC ME | ||
---|---|---|---|---|---|---|---|---|---|---|
#92465 | #198004 | #33647 | #27251 | #182 SE | #377 SE | |||||
Group 1 | 5′ | 3′ | 5′ | 3′ | 5′ | 3′ | ||||
307 | 0.68 | 0.26 | 0.15 | 0.98 | NME | 2.01 | 2.04 | NME | ND | |
308 | 0.70 | 1.04 | 3.38 | 0.41 | ME | Deletion | 27.20 | ME | ND | |
309 | 1.21 | 2.51 | 1.42 | 1.11 | ME | 1.06 | 0.54 | ME | ND | |
310 | 0.06 | 4.31 | 0.68 | 0.93 | ME | 1.06 | 0.92 | ME | ND | |
311 | 1.05 | 1.08 | 1.31 | 1.26 | None | 1.50 | 0.84 | None | ND | |
313 | 0.94 | 0.47 | 1.46 | 1.32 | ME | 1.23 | 0.96 | ME | ND | |
314 | 0.46 | 6.09 | 2.41 | 0.63 | NME | 0.43 | 0.30 | NME | ND | |
315 | 1.09 | 1.87 | 1.12 | 0.98 | None | 9.97 | 0.28 | ME | ND | |
317 | 0.61 | 2.26 | 1.56 | 0.91 | ME | Deletion | 1.40 | NME | ND | |
318 | 1.23 | 0.90 | 5.48 | 2.21 | ME | 0.42 | 0.38 | ME | ND | |
319 | 1.86 | 2.29 | 1.30 | 0.82 | ME | 2.16 | 3.57 | NME | ND | |
321 | 0.77 | 0.29 | 0.69 | 1.03 | ME | 1.26 | 1.74 | None | ND | |
322 | 1.12 | 2.01 | 0.97 | 0.93 | ME | 1.16 | 0.07 | NME | ND | |
323 | 0.86 | 0.52 | 0.75 | 1.06 | None | 1.08 | 1.56 | None | ND | |
324 | 0.64 | 2.96 | 0.77 | 0.16 | NME | 2.31 | 0.31 | NME | ND | |
325 | 1.10 | 1.51 | 2.13 | 0.45 | ME | 0.34 | 0.29 | ME | ND | |
326 | 1.00 | 0.59 | 0.28 | 0.60 | ME | 2.37 | 0.62 | ME | ND | |
327 | 1.32 | >10.0 | 1.04 | 1.03 | ME | 1.80 | No deletion | ME | ND | |
328 | 2.33 | 4.97 | 1.53 | 1.34 | ME | No deletion | No deletion | ME | 2 | NME |
329 | 0.74 | 0.80 | 1.02 | 2.69 | ME | 0.25 | 0.9 | ME | 2 | ME |
330 | 0.87 | 0.72 | 0.96 | 0.64 | None | 0.88 | 13.97 | ME | 1 | None |
331 | 1.11 | 1.08 | 0.10 | 4.66 | ME | 1.34 | 0.48 | ME | 0 | None |
332 | 0.82 | 0.52 | 0.68 | 0.74 | None | 0.97 | 0.40 | ME | 2 | ME |
333 | 1.15 | 0.48 | 0.89 | 1.06 | ME | 1.89 | 1.09 | ME | 1 | ME |
277 | 0.41 | 0.41 | 1.19 | 1.02 | ME | 0.61 | No deletion | ME | 1 | ME |
295 | 0.74 | 0.13 | 1.24 | 1.01 | ME | 0.99 | 1.02 | ME | 1 | ME |
299 | 0.32 | 2.11 | 1.18 | 0.79 | ME | 0.87 | 0.51 | ME | 1 | ME |
338 | 1.73 | 1.27 | 1.54 | 0.04 | ME | 13.1 | 4.06 | ME | 0 | None |
349 | 2.95 | 20.00 | 0.93 | 1.21 | ME | 1.73 | 1.62 | ME | 1 | ME |
351 | 1.69 | 0.41 | No deletion | No deletion | ME | 0.83 | 1.08 | ME | 1 | ME |
379 | 0.98 | 0.18 | 0.76 | 0.64 | ME | 0.92 | 0.94 | ME | 2 | NME |
380 | 0.33 | 0.33 | 0.67 | 0.80 | ME | 0.55 | 1.82 | ME | ND | |
271 | 0.66 | 0.59 | 0.49 | 0.91 | ME | 0.65 | 3.39 | ME | 2 | ME |
272 | 0.87 | 0.92 | 0.78 | 1.93 | None | 0.92 | 0.82 | None | 0 | None |
273 | 0.57 | 0.41 | 0.32 | 0.83 | NME | 0.23 | Deletion | NME | 2 | NME |
274 | 1.02 | 0.87 | 0.70 | 0.95 | None | 0.69 | 0.17 | ME | 1 | None |
275 | 0.55 | 0.90 | 0.18 | 1.04 | ME | 0.35 | Deletion | ME | 2 | ME |
278 | 0.95 | No deletion | 0.76 | 3.11 | ME | 0.56 | 14.00 | ME | 2 | ME |
282 | 1.12 | 1.02 | 1.03 | 0.95 | None | 0.93 | 4.01 | ME | 2 | ME |
284 | 1.02 | 1.02 | 0.35 | 0.92 | ME | 2.27 | 10.40 | ME | 0 | None |
290 | 1.03 | 0.92 | 2.48 | 1.03 | ME | 0.96 | 0.92 | None | 2 | ME |
293 | 0.94 | 0.99 | 0.60 | 0.73 | None | 1.86 | 0.47 | ME | 0 | None |
Group 2 | ||||||||||
269 | 0.74 | 0.74 | 0.76 | 0.15 | ME | 0.71 | 0.33 | ME | 2 | ME |
270 | 1.08 | 1.16 | 1.10 | 0.99 | None | 0.73 | 1.06 | None | 2 | ME |
276 | 0.85 | 1.07 | 4.00 | 1.41 | ME | 0.15 | 3.08 | ME | 2 | ME |
300 | 1.49 | 0.96 | 1.30 | 0.66 | None | 2.23 | 0.57 | ME | 2 | ME |
334 | 0.80 | 0.61 | 0.35 | 0.94 | ME | 1.05 | 4.73 | ME | 2 | ME |
337 | 1.06 | 0.81 | 1.09 | 0.88 | None | 1.66 | 2.07 | ME | 2 | ME |
339 | 0.66 | 1.75 | 1.27 | 0.07 | ME | 0.93 | 0.95 | None | 2 | ME |
340 | 3.90 | 0.91 | 1.30 | 1.25 | ME | 1.71 | No deletion | ME | 1 | ME |
353 | 4.28 | 0.37 | 1.20 | 0.59 | ME | 3.62 | No deletion | NME | 1 | ME |
356 | 1.68 | 1.03 | 1.65 | 0.59 | None | No deletion | No deletion | None | 0 | None |
357 | 1.17 | 1.26 | 1.88 | 0.18 | ME | 2.59 | 2.55 | ME | 1 | None |
358 | 1.07 | 1.23 | 2.91 | 1.45 | ME | 2.01 | 0.65 | ME | 1 | None |
360 | 0.95 | 1.02 | 0.25 | 0.41 | ME | 0.45 | 3.11 | ME | 2 | ME |
362 | 1.64 | No deletion | 1.33 | 1.03 | None | 0.88 | 0.22 | ME | 2 | ME |
363 | 0.84 | 0.88 | 0.27 | 1.22 | ME | 0.35 | 0.18 | ME | 1 | None |
364 | 1.04 | 1.07 | 0.11 | 0.06 | ME | 0.75 | 0.16 | ME | 0 | None |
365 | No deletion | 0.93 | 1.29 | 0.95 | None | 0.75 | 0.93 | None | 1 | None |
366 | 0.87 | 1.26 | 0.44 | 0.99 | ME | 1.04 | 0.98 | None | 2 | ME |
367 | 0.92 | 1.04 | 0.78 | 2.15 | ME | 0.70 | 1.20 | None | 0 | None |
368 | 0.97 | 0.48 | 0.35 | 0.27 | NME | 1.52 | 0.91 | ME | 1 | ME |
369 | 1.49 | 1.06 | 1.40 | 1.27 | None | 3.42 | No deletion | ME | 0 | None |
370 | 0.55 | 1.11 | 1.76 | 0.26 | ME | 0.47 | No deletion | ME | 2 | ME |
371 | 1.49 | 0.56 | 8.11 | 2.46 | ME | 0.71 | 0.25 | ME | 0 | None |
372 | 2.50 | 1.72 | 0.51 | 0.57 | ME | 0.27 | No deletion | NME | 2 | NME |
381 | 1.30 | 0.99 | 1.02 | 1.19 | None | 0.55 | 0.49 | ME | 0 | None |
384 | 1.63 | 0.80 | 2.53 | 0.48 | ME | 1.54 | 1.38 | None | 2 | ME |
387 | 1.23 | 0.26 | 0.39 | 1.99 | NME | 2.04 | 1.80 | NME | 0 | ME |
389 | 1.14 | 1.23 | 1.47 | 0.17 | ME | 1.16 | 0.42 | ME | 1 | None |
Percentage | 26/70 = 37.1% | 33/70 = 47.1% | 47/53 = 88.7% | 43/70 = 61.4% | 48/58 = 82.8% | 29/33 = 87.9% | ||||
p = 0.0023 | p = 0.0128 | |||||||||
X2 = 9.330 | X2 = 6.195 | |||||||||
95% CI | 80.1–97.2% | 95% CI | 75.6–93.9% | 76.7–99.0% |
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Fry, E.A.; Niehans, G.E.; Kratzke, R.A.; Kai, F.; Inoue, K. Survival of Lung Cancer Patients Dependent on the LOH Status for DMP1, ARF, and p53. Int. J. Mol. Sci. 2020, 21, 7971. https://doi.org/10.3390/ijms21217971
Fry EA, Niehans GE, Kratzke RA, Kai F, Inoue K. Survival of Lung Cancer Patients Dependent on the LOH Status for DMP1, ARF, and p53. International Journal of Molecular Sciences. 2020; 21(21):7971. https://doi.org/10.3390/ijms21217971
Chicago/Turabian StyleFry, Elizabeth A., Gloria E. Niehans, Robert A. Kratzke, Fumitake Kai, and Kazushi Inoue. 2020. "Survival of Lung Cancer Patients Dependent on the LOH Status for DMP1, ARF, and p53" International Journal of Molecular Sciences 21, no. 21: 7971. https://doi.org/10.3390/ijms21217971