The Transient Receptor Potential Melastatin 7 (TRPM7) Inhibitors Suppress Seizure-Induced Neuron Death by Inhibiting Zinc Neurotoxicity
Abstract
:1. Introduction
2. Results
2.1. Carvacrol Reduces Seizure-Induced TRPM7 Overexpression, Zinc Accumulation, and Neuronal Death
2.2. Carvacrol Reduces Seizure-Induced Superoxide Production and Oxidative Stress
2.3. Carvacrol Reduces Glial Activation after Pilocarpine-Induced Seizure
2.4. Carvacrol Reduces Seizure-Induced BBB Breakdown and Vessel Disorganization
2.5. Carvacrol Reduces Seizure-Induced Apoptotic Neuronal Death
2.6. 2-APB Reduces Seizure-Induced TRPM7 Overexpression, Zinc Accumulation, and Neuronal Death
3. Discussion
4. Materials and Methods
4.1. Ethics Statement and Experimental Animals
4.2. Seizure Induction
4.3. Experimental Design and Inhibitor Treatment
4.4. Brain Sample Preparation
4.5. Detection of Neuronal Degeneration
4.6. Detection of Zinc Translocation
4.7. Detection of Reactive Oxygen Species (ROS) Activation
4.8. Immunofluorescence Assay
4.9. Immunohistochemistry Assay
4.10. Western Blot
4.11. Data Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Jeong, J.H.; Lee, S.H.; Kho, A.R.; Hong, D.K.; Kang, D.H.; Kang, B.S.; Park, M.K.; Choi, B.Y.; Choi, H.C.; Lim, M.-S.; et al. The Transient Receptor Potential Melastatin 7 (TRPM7) Inhibitors Suppress Seizure-Induced Neuron Death by Inhibiting Zinc Neurotoxicity. Int. J. Mol. Sci. 2020, 21, 7897. https://doi.org/10.3390/ijms21217897
Jeong JH, Lee SH, Kho AR, Hong DK, Kang DH, Kang BS, Park MK, Choi BY, Choi HC, Lim M-S, et al. The Transient Receptor Potential Melastatin 7 (TRPM7) Inhibitors Suppress Seizure-Induced Neuron Death by Inhibiting Zinc Neurotoxicity. International Journal of Molecular Sciences. 2020; 21(21):7897. https://doi.org/10.3390/ijms21217897
Chicago/Turabian StyleJeong, Jeong Hyun, Song Hee Lee, A Ra Kho, Dae Ki Hong, Dong Hyeon Kang, Beom Seok Kang, Min Kyu Park, Bo Young Choi, Hui Chul Choi, Man-Sup Lim, and et al. 2020. "The Transient Receptor Potential Melastatin 7 (TRPM7) Inhibitors Suppress Seizure-Induced Neuron Death by Inhibiting Zinc Neurotoxicity" International Journal of Molecular Sciences 21, no. 21: 7897. https://doi.org/10.3390/ijms21217897