Effects of Detergent on α-Synuclein Structure: A Native MS-Ion Mobility Study
Abstract
:1. Introduction
2. Results
2.1. Interaction of Detergents with α-Synuclein
2.2. Detergent Binding Capacity and Stoichiometry
2.3. Conformational Selectivity of Detergent Binding
2.4. Conformational Effects of Detergent Binding
2.5. Probing Conformational Transitions
2.6. Detergent Concentration Effects
2.7. Effect of Chain Length within the Same Detergent Class
3. Discussion
4. Materials and Methods
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
α-syn | α-synuclein |
aa | amino acid |
PD | Parkinson’s disease |
NAC | Non-Amyloid-β Component |
PTM | Post translational modification |
SMALP | Styrene maleic acid lipid particle |
SUV | Small unilamellar vesicles |
CMC | Critical micelle concentration |
nESI-IM-MS | Nano-electrospray ionisation ion mobility-mass spectrometry |
CCS | Collision cross section |
CIU | Collision induced unfolding |
β-lac | Β-lactoglobulin |
DDM | n-Dodecyl β-D-maltoside |
PC-14 | n-tetradecylphosphocholine |
PC-15 | n-pentadecylphosphocholine |
PC-16 | n-hexadecylphosphocholine |
SDS | sodium dodecyl sulfate |
SCDC | sodium chenodeoxycholate |
SGDC | sodium glycodeoxycholate |
CTAB | Cetrimonium bromide |
SASA | Solvent accessible surface area |
MD | Molecular dynamics |
HD | High definition |
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Name | CMC (mM) | Standard Concentration (mM) | Standard Concentration (× CMC) | Equal Concentration (mM) | Equal Concentration (× CMC) |
---|---|---|---|---|---|
DDM | 0.17 | 0.34 | 2.0 | 0.2 | 1.18 |
PC-14 | 0.12 | 0.24 | 2.0 | 0.2 | 1.67 |
PC-15 L | 0.07 | 0.14 | 2.0 | 0.2 | |
PC-15 H | 0.07 | 0.24 | 3.4 | 0.2 | 2.86 |
PC-16 L | 0.013 | 0.026 | 2.0 | 0.2 | |
PC-16 H | 0.013 | 0.24 | 18.5 | 0.2 | 15.38 |
SDS | 8.2 | 0.164 | 0.02 | 0.2 | 0.024 |
SCDC | 3.0 | 0.6 | 0.2 | 0.2 | 0.067 |
SGDC | 2.1 | 0.42 | 0.2 | 0.2 | 0.095 |
Structure | Name | Type | CMC (mM) | x CMC Used | Molecular Mass (Da) |
---|---|---|---|---|---|
DDM | Non-ionic | 0.170 | 2 | 510.6 | |
Triton X-100 | Non-ionic | 0.230 | 2 | 650 * | |
PC-14 | Zwitterionic | 0.120 | 2 | 379.5 | |
PC-15 | Zwitterionic | 0.070 | 2 & 3.4 | 393.5 | |
PC-16 | Zwitterionic | 0.013 | 2 & 18.5 | 407.5 | |
SCDC | Anionic | 3.000 | 0.2 | 414.6 | |
SGDC | Anionic | 2.100 | 0.2 | 471.6 | |
SDS | Anionic | 8.200 | 0.02 | 288.4 | |
CTAB | Cationic | 0.920 | 0.2 | 364.5 |
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Moons, R.; van der Wekken-de Bruijne, R.; Maudsley, S.; Lemière, F.; Lambeir, A.-M.; Sobott, F. Effects of Detergent on α-Synuclein Structure: A Native MS-Ion Mobility Study. Int. J. Mol. Sci. 2020, 21, 7884. https://doi.org/10.3390/ijms21217884
Moons R, van der Wekken-de Bruijne R, Maudsley S, Lemière F, Lambeir A-M, Sobott F. Effects of Detergent on α-Synuclein Structure: A Native MS-Ion Mobility Study. International Journal of Molecular Sciences. 2020; 21(21):7884. https://doi.org/10.3390/ijms21217884
Chicago/Turabian StyleMoons, Rani, Renate van der Wekken-de Bruijne, Stuart Maudsley, Filip Lemière, Anne-Marie Lambeir, and Frank Sobott. 2020. "Effects of Detergent on α-Synuclein Structure: A Native MS-Ion Mobility Study" International Journal of Molecular Sciences 21, no. 21: 7884. https://doi.org/10.3390/ijms21217884