Doxorubicin Improves Cancer Cell Targeting by Filamentous Phage Gene Delivery Vectors
Abstract
:1. Introduction
2. Results
2.1. Doxorubicin Increases Targeted Gene Transfer by RGD4C/AAVP in Rat Glioma (9L) and Human Melanoma (M21) Cancer Cells In Vitro
2.2. Doxorubicin Drug Treatment Boosts Cancer Cell Death by AAVP-Mediated Suicide Gene Killing
2.3. Evaluation of the Effect of Doxorubicin Treatment on Vector Cellular Trafficking
2.4. Evaluation of Efficacy of Doxorubicin and RGD4C/AAVP Combination in a Three-Dimensional (3D) Multicellular Tumor Spheroid Model
3. Discussion
4. Materials and Methods
4.1. Cells and Reagents
4.2. Production, Purification and Titration of AAVP Vectors
4.3. In Vitro Cell Transduction by AAVP Vectors
4.4. Reporter Gene Assays
4.5. Determination of Tumor Cell Killing In Vitro
4.6. D Model of Multicellular Tumor Spheroid Culture and Treatment
4.7. Attachment Assay
4.8. Internalization Assay
4.9. Nuclei Extraction
4.10. Semi-Quantitative PCR Analysis
4.11. Comet Assay
4.12. Immunofluorescence Staining for Lamin B1
4.13. Western Blot for Lamin B1
4.14. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Tsafa, E.; Bentayebi, K.; Topanurak, S.; Yata, T.; Przystal, J.; Fongmoon, D.; Hajji, N.; Waramit, S.; Suwan, K.; Hajitou, A. Doxorubicin Improves Cancer Cell Targeting by Filamentous Phage Gene Delivery Vectors. Int. J. Mol. Sci. 2020, 21, 7867. https://doi.org/10.3390/ijms21217867
Tsafa E, Bentayebi K, Topanurak S, Yata T, Przystal J, Fongmoon D, Hajji N, Waramit S, Suwan K, Hajitou A. Doxorubicin Improves Cancer Cell Targeting by Filamentous Phage Gene Delivery Vectors. International Journal of Molecular Sciences. 2020; 21(21):7867. https://doi.org/10.3390/ijms21217867
Chicago/Turabian StyleTsafa, Effrosyni, Kaoutar Bentayebi, Supachai Topanurak, Teerapong Yata, Justyna Przystal, Duriya Fongmoon, Nabil Hajji, Sajee Waramit, Keittisak Suwan, and Amin Hajitou. 2020. "Doxorubicin Improves Cancer Cell Targeting by Filamentous Phage Gene Delivery Vectors" International Journal of Molecular Sciences 21, no. 21: 7867. https://doi.org/10.3390/ijms21217867