BacSJ—Another Bacteriocin with Distinct Spectrum of Activity that Targets Man-PTS
Abstract
:1. Introduction
2. Results
2.1. BacSJ Homologs are Encoded in Some Lactobacillales
2.2. BacSJ Has a Moderate Spectrum of Activity
2.3. Man-PTS Subunits IIC and IID are Necessary for BacSJ Activity
2.4. Targeted Mutagenesis Allows Identification of Man-PTS Amino Acids Needed for BacSJ Activity
3. Discussion
4. Materials and Methods
4.1. Bacterial and Yeast Strains and Growth Conditions
4.2. Bacteriocin Preparation
4.3. Activity Spectrum Assay and Selection of Resistant Mutants
4.4. DNA Isolation and Manipulation
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Group | Indicator Strain | BacSJ Activity |
---|---|---|
Fungi | Candida albicans CAI-4 | − |
G− | Campylobacter jejuni 12 | − |
G− | Campylobacter jejuni 480 | − |
G− | Campylobacter jejuni 81176 | − |
G− | Campylobacter coli 23/1 | − |
G− | Escherichia coli EC1000 | − |
G− | Escherichia coli TG1 | − |
G− | Pseudomonas aeruginosa ATCC 9027 | − |
G− | Salmonella typhimurium TT622 | − |
G+ | Bacillus cereus IBB3390 | − |
G+ | Bacillus subtilis BSB1 | − |
G+ | Carnobacterium maltaromaticum IBB3447 | + |
G+ | Enterococcus durans IBB3441 | + |
G+ | Enterococcus faecalis IBB3439 | + |
G+ | Enterococcus faecalis IBB3444 | + |
G+ | Enterococcus faecium LMGT 2783 | + |
G+ | Enterococcus faecium LMGT 2787 | + |
G+ | Lactobacillus johnsonii IBB3155 | +/− |
G+ | Lactobacillus kunkeei AH1 | − |
G+ | Lactobacillus kunkeei AH38 | − |
G+ | Lactobacillus kunkeei AH119 | − |
G+ | Lactobacillus paracasei IBB3418 | + |
G+ | Lactobacillus paracasei IBB3425 | + |
G+ | Lactobacillus paracasei IBB3426 | + |
G+ | Lactobacillus paracasei IBB3427 | + |
G+ | Lactobacillus paracasei IBB3428 | + |
G+ | Lactobacillus paracasei LOCK 0919 | + |
G+ | Lactobacillus paracasei subsp. paracasei IBB3423 | + |
G+ | Lactobacillus paraplantarum IBB3438 | + |
G+ | Lactobacillus plantarum NC8 | + |
G+ | Lactobacillus plantarum WCSF1 | + |
G+ | Lactobacillus plantarum IBB3433 | + |
G+ | Lactobacillus plantarum IBB3436 | + |
G+ | Lactobacillus plantarum subsp. plantarum IBB3434 | + |
G+ | Lactobacillus rhamnosus GG | + |
G+ | Lactobacillus rhamnosus IBB3429 | + |
G+ | Lactobacillus rhamnosus LOCK 0900 | ++ |
G+ | Lactobacillus rhamnosus LOCK 0908 | + |
G+ | Lactobacillus salivarius IBB3154 | + |
G+ | Lactococcus garvieae IBB3403 | − |
G+ | Lactococcus garvieae IBB66 | − |
G+ | Lactococcus lactis IBB3404 | + |
G+ | Lactococcus lactis IBB3411 | + |
G+ | Lactococcus lactis QU5 LMGT 3419 | + |
G+ | Lactococcus lactis subsp. cremoris IBB3409 | + |
G+ | Lactococcus lactis subsp. lactis IBB3407 | + |
G+ | Lactococcus lactis subsp. lactis IL1403 | + |
G+ | Lactococcus raffinolactis IBB91 | + |
G+ | Lactococcus lactis IBB3446 | + |
G+ | Leuconostoc mesenteroides IBB3442 | + |
G+ | Leuconostoc mesenteroides IBB3443 | + |
G+ | Listeria monocytogenes EDG-e | + |
G+ | Pediococcus acidilactici LMGT 2002 | − |
G+ | Pediococcus parvulus IBB3448 | − |
G+ | Pediococcus pentosaceus IBB3369 | + |
G+ | Staphylococcus aureus ATCC 6538 | − |
G+ | Staphylococcus caprae DSM-20608 | − |
G+ | Staphylococcus delphini DSM-20771 | − |
G+ | Staphylococcus epidermidis DSM-20044 | − |
G+ | Staphylococcus hyicus DSM-20459 | − |
G+ | Staphylococcus intermedius DSM-20373 | − |
G+ | Staphylococcus lugdunensis DSM-4804 | − |
G+ | Staphylococcus pseudintermedius DSM-21284 | − |
G+ | Staphylococcus saprophyticus DSM-18669 | − |
G+ | Staphylococcus schleiferi DSM-6628 | − |
G+ | Streptococcus agalactiae IBB123 | − |
G+ | Streptococcuss agalactiae IBB130 | − |
G+ | Streptococcus mitis IBB3449 | − |
G+ | Streptococcus parauberis IBB272 | − |
G+ | Streptococcus sobrinus IBB3450 | − |
Mutant | Mutation | Amino Acid Change | Sensitivity to (Fold-Decreased Relative to WT) | Position in the Cell Membrane of Substituted Man-PTS Amino Acid | |
---|---|---|---|---|---|
GarQ | BacSJ | ||||
GarQ-resistant mutant | |||||
LLN1 | C368→ A in ptnD | Pro123 → His | >1024× | >8× | outside |
BacSJ-resistant mutants | |||||
M3, M8, M9 | G185 → T in ptnC | Gly62→Val | 64× | >8× | inside |
M6, M33 | G599→ A in ptnD | Arg200 → His | 8× | >8× | outside |
M16 | C247→T in ptnD | Leu83→Phe | 0× | >8× | outside |
M19 | T677→C in ptnD | Phe226→Ser | 8× | >8× | outside |
M30 | G591→ T in ptnD | Leu197→Phe | 16× | >8× | transmembrane |
Strain | IIC | IID | ||||
---|---|---|---|---|---|---|
BacSJ sensitive | L. lactis IL1403 | Gly62 i | Leu83 o | Leu197 t | Arg200 o | Phe226 o |
L. lactis IBB3407 | Gly62 i | Leu83 o | Leu197 t | Arg200 o | Phe226 o | |
L. lactis IBB3409 | Gly62 i | Leu83 o | Leu197 t | Arg200 o | Phe226 o | |
L. lactis IBB2955 | Gly62 i | Leu83 o | Leu197 t | Arg200 o | Phe226 o | |
L. lactis LMGT3419 | Gly62 i | Leu83 o | Leu197 t | Arg200 o | Phe226 o | |
L. paracasei IBB3418 | Gly62 i | Leu82 o | Leu196 o | Arg199 o | Trp222 o | |
L. paracasei IBB3424 | Gly62 i | Leu82o | Leu196o | Arg199o | Trp222o | |
L. paracasei IBB3427 | Gly62 i | Leu82 o | Leu196 o | Arg199 o | Trp222 o | |
L. paracasei LOCK 0919 | Gly62 i | Leu82 o | Leu196 o | Arg199 o | Trp222 o | |
L. paraplantarum IBB3438 | Gly62 i | Leu83 o | Leu197 t | Arg200 o | Trp224 o | |
L. plantarum IBB3036 | Gly62 i | Leu88 o | Leu202 o | Arg205 o | Trp229 o | |
L. plantarum IBB3434 | Gly62 i | Leu88 o | Leu202 t | Arg205 o | Trp229 o | |
L. plantarum IBB3436 | Gly62 i | Leu83 o | Leu197 t | Arg200 o | Trp224 o | |
L. plantarum NC8 | Gly62 i | Leu88 o | Leu202 o | Arg205 o | Trp229 o | |
L. plantarum WCFS1 | Gly62 i | Leu83 o | Leu197 t | Arg200 o | Trp224 o | |
L. rhamnosus GG | Gly62 i | Leu82 o | Leu196 o | Arg199 o | Trp222 o | |
L. rhamnosus IBB3429 | Gly62 i | Leu82 o | Leu196 o | Arg199 o | Trp222 o | |
L. rhamnosus LOCK 0900 | Gly62 i | Leu82 o | Leu196 o | Arg199 o | Trp222 o | |
L. rhamnosus LOCK 0908 | Gly62 i | Leu82 o | Leu196 o | Arg199 o | Trp222 o | |
L. salivarius IBB3154 | Gly60 i | Leu83 o | Leu197 o | Arg200 o | Trp223 o | |
E. faecium LMGT2783 | Gly60 i | Leu83 o | Leu197 o | Arg200 o | Trp223 o | |
L. monocytogenes EGD-e | Gly60 i | Leu82 o | Leu196 t | Arg199 o | Trp222 o | |
BacSJ resistant | L. garvieae IBB66 | Gly60 i | Leu86 o | Leu200 t | Arg203 o | Trp230 o |
L. garvieae IBB3403 | Gly60 i | Leu86 o | Leu200 t | Arg203 o | Trp230 o | |
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Tymoszewska, A.; Walczak, P.; Aleksandrzak-Piekarczyk, T. BacSJ—Another Bacteriocin with Distinct Spectrum of Activity that Targets Man-PTS. Int. J. Mol. Sci. 2020, 21, 7860. https://doi.org/10.3390/ijms21217860
Tymoszewska A, Walczak P, Aleksandrzak-Piekarczyk T. BacSJ—Another Bacteriocin with Distinct Spectrum of Activity that Targets Man-PTS. International Journal of Molecular Sciences. 2020; 21(21):7860. https://doi.org/10.3390/ijms21217860
Chicago/Turabian StyleTymoszewska, Aleksandra, Piotr Walczak, and Tamara Aleksandrzak-Piekarczyk. 2020. "BacSJ—Another Bacteriocin with Distinct Spectrum of Activity that Targets Man-PTS" International Journal of Molecular Sciences 21, no. 21: 7860. https://doi.org/10.3390/ijms21217860