Mitochondrial Function and Protein Turnover in the Diaphragm are Altered in LLC Tumor Model of Cancer Cachexia
Abstract
:1. Introduction
2. Results
2.1. Lewis Lung Carcinoma (LLC) Resulted in Alterations to Mitochondrial Function without Changes to Mitochondrial Content
2.2. LLC Resulted in Greater Reactive Oxgyen Species Generation without any Changes in most Antioxidant Related Proteins
2.3. LLC Did not Have Significant Effects on Protein Synthesis Signaling
2.4. LLC Appeared to Increase FOXO3-Dependent Signaling
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Study Design
4.3. Determination of Muscle Protein Synthesis
4.4. Mitochondrial Function
4.5. Reactive Oxygen Species (ROS) Emission
4.6. Western Blotting
4.7. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Rosa-Caldwell, M.E.; Benson, C.A.; Lee, D.E.; Brown, J.L.; Washington, T.A.; Greene, N.P.; Wiggs, M.P. Mitochondrial Function and Protein Turnover in the Diaphragm are Altered in LLC Tumor Model of Cancer Cachexia. Int. J. Mol. Sci. 2020, 21, 7841. https://doi.org/10.3390/ijms21217841
Rosa-Caldwell ME, Benson CA, Lee DE, Brown JL, Washington TA, Greene NP, Wiggs MP. Mitochondrial Function and Protein Turnover in the Diaphragm are Altered in LLC Tumor Model of Cancer Cachexia. International Journal of Molecular Sciences. 2020; 21(21):7841. https://doi.org/10.3390/ijms21217841
Chicago/Turabian StyleRosa-Caldwell, Megan E., Conner A. Benson, David E. Lee, Jacob L. Brown, Tyrone A. Washington, Nicholas P. Greene, and Michael P. Wiggs. 2020. "Mitochondrial Function and Protein Turnover in the Diaphragm are Altered in LLC Tumor Model of Cancer Cachexia" International Journal of Molecular Sciences 21, no. 21: 7841. https://doi.org/10.3390/ijms21217841