Overexpression of PtrMYB121 Positively Regulates the Formation of Secondary Cell Wall in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Phylogenetic Analysis and Amino Acid Sequence Alignment of PtrMYB121
2.2. Tissue Expression Pattern Analysis of PtrMYB55 and PtrMYB121
2.3. Overexpression of PtrMYB121 in A. thaliana
2.4. Overexpression of PtrMYB121 Promotes the Accumulation of Lignin and Cellulose
2.5. Overexpression of PtrMYB121 Activates the Expression of SCW Biosynthetic Genes
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Phylogenetic Analysis
4.3. RNA Isolation and qRT-PCR Analysis
4.4. Cloning of Full-Length PtrMYB121 Coding Sequence (CDS)
4.5. Arabidopsis Transformation
4.6. Phenotypic Observation of PtrMYB121-Overexpressing Lines in Arabidopsis
4.7. Measurement of Lignin, Cellulose, and Hemicellulose Components in Arabidopsis
4.8. LUC Activity Assay
4.9. Statistical Analysis
4.10. GenBank Accession Numbers of Genes Used in this Study
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Line | Samples | Cell Layer (n) | Radial Width (μm) |
---|---|---|---|
WT-1 | 1 | 6.78 ± 0.81 a | 439.72 ± 28.79 a |
2 | 7.11 ± 0.94 a | 461.77 ± 33.47 a | |
3 | 6.43 ± 0.87 a | 422.36 ± 30.16 a | |
PtrMYB121-L1 | 1 | 13.76 ± 1.57 b | 657.42 ± 53.34 b |
2 | 14.20 ± 1.72 b | 693.17 ± 58.38 b | |
3 | 13.49 ± 1.55 b | 638.84 ± 49.67 b |
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Liu, Y.; Man, J.; Wang, Y.; Yuan, C.; Shi, Y.; Liu, B.; Hu, X.; Wu, S.; Zhang, T.; Lian, C. Overexpression of PtrMYB121 Positively Regulates the Formation of Secondary Cell Wall in Arabidopsis thaliana. Int. J. Mol. Sci. 2020, 21, 7734. https://doi.org/10.3390/ijms21207734
Liu Y, Man J, Wang Y, Yuan C, Shi Y, Liu B, Hu X, Wu S, Zhang T, Lian C. Overexpression of PtrMYB121 Positively Regulates the Formation of Secondary Cell Wall in Arabidopsis thaliana. International Journal of Molecular Sciences. 2020; 21(20):7734. https://doi.org/10.3390/ijms21207734
Chicago/Turabian StyleLiu, Ying, Jiayin Man, Yinghao Wang, Chao Yuan, Yuyu Shi, Bobin Liu, Xia Hu, Songqing Wu, Taoxiang Zhang, and Chunlan Lian. 2020. "Overexpression of PtrMYB121 Positively Regulates the Formation of Secondary Cell Wall in Arabidopsis thaliana" International Journal of Molecular Sciences 21, no. 20: 7734. https://doi.org/10.3390/ijms21207734