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Open AccessArticle

Roles of ABCC1 and ABCC4 in Proliferation and Migration of Breast Cancer Cell Lines

College of Health & Life Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(20), 7664; https://doi.org/10.3390/ijms21207664
Received: 18 September 2020 / Revised: 6 October 2020 / Accepted: 7 October 2020 / Published: 16 October 2020
(This article belongs to the Special Issue ABC Transporters in Human Diseases)
ABCC1 and ABCC4 utilize energy from ATP hydrolysis to transport many different molecules, including drugs, out of the cell and, as such, have been implicated in causing drug resistance. However recently, because of their ability to transport signaling molecules and inflammatory mediators, it has been proposed that ABCC1 and ABCC4 may play a role in the hallmarks of cancer development and progression, independent of their drug efflux capabilities. Breast cancer is the most common cancer affecting women. In this study, the aim was to investigate whether ABCC1 or ABCC4 play a role in the proliferation or migration of breast cancer cell lines MCF-7 (luminal-type, receptor-positive) and MDA-MB-231 (basal-type, triple-negative). The effects of small molecule inhibitors or siRNA-mediated knockdown of ABCC1 or ABCCC4 were measured. Colony formation assays were used to assess the clonogenic capacity, MTT assays to measure the proliferation, and scratch assays and Transwell assays to monitor the cellular migration. The results showed a role for ABCC1 in cellular proliferation, whilst ABCC4 appeared to be more important for cellular migration. ELISA studies implicated cAMP and/or sphingosine-1-phosphate efflux in the mechanism by which these transporters mediate their effects. However, this needs to be investigated further, as it is key to understand the mechanisms before they can be considered as targets for treatment. View Full-Text
Keywords: MRP1; MRP4; breast cancer; proliferation; migration; invasion; cAMP MRP1; MRP4; breast cancer; proliferation; migration; invasion; cAMP
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MDPI and ACS Style

Low, F.G.; Shabir, K.; Brown, J.E.; Bill, R.M.; Rothnie, A.J. Roles of ABCC1 and ABCC4 in Proliferation and Migration of Breast Cancer Cell Lines. Int. J. Mol. Sci. 2020, 21, 7664. https://doi.org/10.3390/ijms21207664

AMA Style

Low FG, Shabir K, Brown JE, Bill RM, Rothnie AJ. Roles of ABCC1 and ABCC4 in Proliferation and Migration of Breast Cancer Cell Lines. International Journal of Molecular Sciences. 2020; 21(20):7664. https://doi.org/10.3390/ijms21207664

Chicago/Turabian Style

Low, Floren G.; Shabir, Kiran; Brown, James E.; Bill, Roslyn M.; Rothnie, Alice J. 2020. "Roles of ABCC1 and ABCC4 in Proliferation and Migration of Breast Cancer Cell Lines" Int. J. Mol. Sci. 21, no. 20: 7664. https://doi.org/10.3390/ijms21207664

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