Next Article in Journal
Unveiling the Molecular Basis of the Noonan Syndrome-Causing Mutation T42A of SHP2
Previous Article in Journal
Supporting Cell-Based Tendon Therapy: Effect of PDGF-BB and Ascorbic Acid on Rabbit Achilles Tenocytes In Vitro
Open AccessArticle

Divergent Evolution of Eukaryotic CC- and A-Adding Enzymes

1
Institute for Biochemistry, Leipzig University, Brüderstraße 34, 04103 Leipzig, Germany
2
Computational EvoDevo Group, Department of Computer Science, Leipzig University, Härtelstraße 16-18, 04107 Leipzig, Germany
3
Interdisciplinary Center for Bioinformatics, Leipzig University, Härtelstraße 16-18, 04107 Leipzig, Germany
4
Santa Fe Institute for Complex Systems, 1399 Hyde Park Road, Santa Fe, NM 87501, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(2), 462; https://doi.org/10.3390/ijms21020462
Received: 20 December 2019 / Revised: 6 January 2020 / Accepted: 9 January 2020 / Published: 10 January 2020
(This article belongs to the Section Molecular Biology)
Synthesis of the CCA end of essential tRNAs is performed either by CCA-adding enzymes or as a collaboration between enzymes restricted to CC- and A-incorporation. While the occurrence of such tRNA nucleotidyltransferases with partial activities seemed to be restricted to Bacteria, the first example of such split CCA-adding activities was reported in Schizosaccharomyces pombe. Here, we demonstrate that the choanoflagellate Salpingoeca rosetta also carries CC- and A-adding enzymes. However, these enzymes have distinct evolutionary origins. Furthermore, the restricted activity of the eukaryotic CC-adding enzymes has evolved in a different way compared to their bacterial counterparts. Yet, the molecular basis is very similar, as highly conserved positions within a catalytically important flexible loop region are missing in the CC-adding enzymes. For both the CC-adding enzymes from S. rosetta as well as S. pombe, introduction of the loop elements from closely related enzymes with full activity was able to restore CCA-addition, corroborating the significance of this loop in the evolution of bacterial as well as eukaryotic tRNA nucleotidyltransferases. Our data demonstrate that partial CC- and A-adding activities in Bacteria and Eukaryotes are based on the same mechanistic principles but, surprisingly, originate from different evolutionary events. View Full-Text
Keywords: tRNA nucleotidyltransferase; enzyme evolution; Salpingoeca rosetta; Schizosaccharomyces pombe tRNA nucleotidyltransferase; enzyme evolution; Salpingoeca rosetta; Schizosaccharomyces pombe
Show Figures

Figure 1

MDPI and ACS Style

Erber, L.; Franz, P.; Betat, H.; Prohaska, S.; Mörl, M. Divergent Evolution of Eukaryotic CC- and A-Adding Enzymes. Int. J. Mol. Sci. 2020, 21, 462.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop