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Article

In Vitro Maturation with Leukemia Inhibitory Factor Prior to the Vitrification of Bovine Oocytes Improves Their Embryo Developmental Potential and Gene Expression in Oocytes and Embryos

1
Department of Animal Medicine and Surgery, Autonomous University of Barcelona, ES-08193 Cerdanyola del Vallès, Spain
2
Department of Animal Health and Anatomy, Autonomous University of Barcelona, ES-08193 Cerdanyola del Vallès, Spain
3
College of Veterinary Medicine, Western University of Health Sciences, Pomona, CA 91766, USA
4
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada
5
Department of Biology, Institute of Food and Agricultural Technology, University of Girona, ES-17004 Girona, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(19), 7067; https://doi.org/10.3390/ijms21197067
Received: 17 May 2020 / Revised: 16 September 2020 / Accepted: 22 September 2020 / Published: 25 September 2020
(This article belongs to the Special Issue Cryopreservation of Gametes and Embryos)
Oocyte cryopreservation has a significant impact on subsequent embryonic development. Herein, we investigated whether supplementing in vitro maturation medium with Leukemia Inhibitory Factor (LIF) prior to vitrification affects embryo development and gene expression at different embryo developmental stages. A panel of genes including maternal effect, epigenetics, apoptosis and heat stress was relatively quantified. The results show reduced cleavage rates after vitrification, regardless of the LIF treatment. Although not statistically different from control-vitrified oocytes, oocyte apoptosis and the blastocyst yield of LIF-vitrified oocytes were similar to their non-vitrified counterparts. Vitrification increased oocyte ZAR1, NPM2 and DPPA3 gene expression while its expression decreased in LIF-vitrified oocytes to similar or close levels to those of non-vitrified oocytes. With a few gene-specific exceptions, vitrification significantly increased the expression of DNMT3A, HDAC1, KAT2A, BAX and BCL2L1 in oocytes and most stages of embryo development, while comparable expression patterns for these genes were observed between LIF-vitrified and non-vitrified groups. Vitrification increased HSPA1A expression in oocytes and HSP90AA1 in 2-cell embryos. Our data suggest that vitrification triggers stage-specific changes in gene expression throughout embryonic development. However, the inclusion of LIF in the IVM medium prior to vitrification stimulates blastocyst development and several other developmental parameters and induces oocytes and embryos to demonstrate gene expression patterns similar to those derived from non-vitrified oocytes. View Full-Text
Keywords: oocyte; cryopreservation; cow; interleukin; cytokine; maternal effect gene; epigenetics; apoptosis; heat shock protein; maternal–embryonic transition; cell count; apoptosis; inner cell mass oocyte; cryopreservation; cow; interleukin; cytokine; maternal effect gene; epigenetics; apoptosis; heat shock protein; maternal–embryonic transition; cell count; apoptosis; inner cell mass
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MDPI and ACS Style

Vendrell-Flotats, M.; García-Martínez, T.; Martínez-Rodero, I.; Lopez-Bejar, M.; LaMarre, J.; Yeste, M.; Mogas, T. In Vitro Maturation with Leukemia Inhibitory Factor Prior to the Vitrification of Bovine Oocytes Improves Their Embryo Developmental Potential and Gene Expression in Oocytes and Embryos. Int. J. Mol. Sci. 2020, 21, 7067. https://doi.org/10.3390/ijms21197067

AMA Style

Vendrell-Flotats M, García-Martínez T, Martínez-Rodero I, Lopez-Bejar M, LaMarre J, Yeste M, Mogas T. In Vitro Maturation with Leukemia Inhibitory Factor Prior to the Vitrification of Bovine Oocytes Improves Their Embryo Developmental Potential and Gene Expression in Oocytes and Embryos. International Journal of Molecular Sciences. 2020; 21(19):7067. https://doi.org/10.3390/ijms21197067

Chicago/Turabian Style

Vendrell-Flotats, Meritxell, Tania García-Martínez, Iris Martínez-Rodero, Manel Lopez-Bejar, Jonathan LaMarre, Marc Yeste, and Teresa Mogas. 2020. "In Vitro Maturation with Leukemia Inhibitory Factor Prior to the Vitrification of Bovine Oocytes Improves Their Embryo Developmental Potential and Gene Expression in Oocytes and Embryos" International Journal of Molecular Sciences 21, no. 19: 7067. https://doi.org/10.3390/ijms21197067

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