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Article

Advantages and Pitfalls of Capillary Electrophoresis of Pharmaceutical Compounds and Their Enantiomers in Complex Samples: Comparison of Hydrodynamically Opened and Closed Systems

1
Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská Dolina CH2, Ilkovičova 6, SK-84215 Bratislava, Slovakia
2
Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, Universitätsplatz 1, A-8010 Graz, Austria
3
Pfizer R & D UK Limited, Ramsgate Road, Sandwich CT13 9NJ, UK
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(18), 6852; https://doi.org/10.3390/ijms21186852
Received: 4 September 2020 / Revised: 15 September 2020 / Accepted: 16 September 2020 / Published: 18 September 2020
(This article belongs to the Special Issue Chemistry towards Biology)
Several research disciplines require fast, reliable and highly automated determination of pharmaceutically active compounds and their enantiomers in complex biological matrices. To address some of the challenges of Capillary Electrophoresis (CE), such as low concentration sensitivity and performance degradation linked to the adsorption and interference of matrix components, CE in a hydrodynamically closed system was evaluated using the model compounds Pindolol and Propranolol. Some established validation parameters such as repeatability of injection efficiency, resolution and sensitivity were used to assess its performance, and it was found to be broadly identical to that of hydrodynamically opened systems. While some reduction in separation efficiency was observed, this was mainly due to dispersion caused by injection and it had no impact on the ability to resolve enantiomers of model compounds even when spiked into complex biological matrix such as blood serum. An approximately 18- to 23-fold increase in concentration sensitivity due to the employment of wide bore capillaries was observed. This brings the sensitivity of CE to a level similar to that of liquid chromatography techniques. In addition to this benefit and unlike in hydrodynamically opened systems, suppression of electroosmotic flow, which is essential for hydrodynamically closed systems practically eliminates the matrix effects that are linked to protein adsorption. View Full-Text
Keywords: Capillary electrophoresis; hydrodynamically opened and closed systems; chiral separation; pharmaceutical analysis; serum analysis Capillary electrophoresis; hydrodynamically opened and closed systems; chiral separation; pharmaceutical analysis; serum analysis
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MDPI and ACS Style

Masár, M.; Hradski, J.; Schmid, M.G.; Szucs, R. Advantages and Pitfalls of Capillary Electrophoresis of Pharmaceutical Compounds and Their Enantiomers in Complex Samples: Comparison of Hydrodynamically Opened and Closed Systems. Int. J. Mol. Sci. 2020, 21, 6852. https://doi.org/10.3390/ijms21186852

AMA Style

Masár M, Hradski J, Schmid MG, Szucs R. Advantages and Pitfalls of Capillary Electrophoresis of Pharmaceutical Compounds and Their Enantiomers in Complex Samples: Comparison of Hydrodynamically Opened and Closed Systems. International Journal of Molecular Sciences. 2020; 21(18):6852. https://doi.org/10.3390/ijms21186852

Chicago/Turabian Style

Masár, Marián, Jasna Hradski, Martin G. Schmid, and Roman Szucs. 2020. "Advantages and Pitfalls of Capillary Electrophoresis of Pharmaceutical Compounds and Their Enantiomers in Complex Samples: Comparison of Hydrodynamically Opened and Closed Systems" International Journal of Molecular Sciences 21, no. 18: 6852. https://doi.org/10.3390/ijms21186852

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