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Cannabidiol Modifies the Formation of NETs in Neutrophils of Psoriatic Patients

Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, Poland
Department of Immunology, Medical University of Bialystok, Waszyngtona 15A, 15-269 Bialystok, Poland
Dermatological Specialized Center “DERMAL” NZOZ in Bialystok, 15-453 Bialystok, Poland
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(18), 6795;
Received: 15 August 2020 / Revised: 11 September 2020 / Accepted: 15 September 2020 / Published: 16 September 2020
(This article belongs to the Special Issue Therapy and Prevention of Atopic Dermatitis and Psoriasis 2020)
Psoriasis is associated with increased production of reactive oxygen species which leads to oxidative stress. As antioxidants can provide protection, the aim of this study was to evaluate the effects of cannabidiol (CBD) on neutrophil extracellular trap (NET) formation in psoriatic and healthy neutrophils. Important markers of NETosis were measured in healthy and psoriatic neutrophils after incubation with CBD, lipopolysaccharide (LPS), and LPS + CBD). The percentage of neutrophils undergoing NETosis and the level of NETosis markers (cfDNA, MPO, elastase) were higher in the neutrophils and blood plasma of psoriatic patients, compared to controls. After LPS treatment, all of the markers of NETosis, except elastase, and p47 and citrullinated histones, were increased in samples from healthy subjects and psoriasis patients. CBD reduced the concentrations of NETosis markers. This led to a reduction in NETosis, which was more pronounced in psoriatic neutrophils and neutrophils treated with LPS in both psoriatic and healthy participants. These results suggest that psoriatic patients neutrophils are at a higher risk of NETosis both in vitro and in vivo. CBD reduces NETosis, mainly in psoriatic neutrophils, possibly due to its antioxidant properties. The anti-NET properties of CBD suggest the positive effect of CBD in the treatment of autoimmune diseases. View Full-Text
Keywords: psoriasis; cannabidiol treatment; neutrophil traps; NETs; immunity psoriasis; cannabidiol treatment; neutrophil traps; NETs; immunity
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MDPI and ACS Style

Wójcik, P.; Garley, M.; Wroński, A.; Jabłońska, E.; Skrzydlewska, E. Cannabidiol Modifies the Formation of NETs in Neutrophils of Psoriatic Patients. Int. J. Mol. Sci. 2020, 21, 6795.

AMA Style

Wójcik P, Garley M, Wroński A, Jabłońska E, Skrzydlewska E. Cannabidiol Modifies the Formation of NETs in Neutrophils of Psoriatic Patients. International Journal of Molecular Sciences. 2020; 21(18):6795.

Chicago/Turabian Style

Wójcik, Piotr, Marzena Garley, Adam Wroński, Ewa Jabłońska, and Elżbieta Skrzydlewska. 2020. "Cannabidiol Modifies the Formation of NETs in Neutrophils of Psoriatic Patients" International Journal of Molecular Sciences 21, no. 18: 6795.

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