F-Box Gene D5RF Is Regulated by Agrobacterium Virulence Protein VirD5 and Essential for Agrobacterium-Mediated Plant Transformation
Abstract
:1. Introduction
2. Results
2.1. The Arabidopsis D5RF Gene Is Regulated by VirD5 at the Transcription Level
2.2. Bioinformatics Analysis and Subcellular Localization of D5RF
2.3. VirD5 Can Activate the Promotor of D5RF.1
2.4. D5RF Is Required for Plant Stable Transformation, But Not for Transient Transformation
2.5. Pathogen-Responsive Genes Are Possibly Downregulated in d5rf
3. Discussions
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Yeast One-Hybrid Assay
4.3. Dual-Luciferase Reporter Assay System
4.4. Agrobacterium-Mediated Plant Stable and Transient Transformation Assay
4.5. Subcellular Localization Assay in Arabidopsis Protoplasts
4.6. Reverse Transcription PCR and RNA-Sequencing
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ABA | Abscisic Acid |
DEGs | Differentially Expressed Genes |
D5RE | VirD5 response element |
D5RF | VirD5 response F-box protein |
RT-PCR | Reverse Transcription-Polymerase Chain Reaction |
RT-QPCR | Real-time quantitative PCR |
WT | Wild Type |
YFP | Yellow Fluorescent Protein |
Y2H | Yeast Two-Hybrid |
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Zhang, S.; Chen, Z.; Huang, F.; Wang, Y.; Luo, M. F-Box Gene D5RF Is Regulated by Agrobacterium Virulence Protein VirD5 and Essential for Agrobacterium-Mediated Plant Transformation. Int. J. Mol. Sci. 2020, 21, 6731. https://doi.org/10.3390/ijms21186731
Zhang S, Chen Z, Huang F, Wang Y, Luo M. F-Box Gene D5RF Is Regulated by Agrobacterium Virulence Protein VirD5 and Essential for Agrobacterium-Mediated Plant Transformation. International Journal of Molecular Sciences. 2020; 21(18):6731. https://doi.org/10.3390/ijms21186731
Chicago/Turabian StyleZhang, Shaojuan, Zhuo Chen, Fei Huang, Yafei Wang, and Meizhong Luo. 2020. "F-Box Gene D5RF Is Regulated by Agrobacterium Virulence Protein VirD5 and Essential for Agrobacterium-Mediated Plant Transformation" International Journal of Molecular Sciences 21, no. 18: 6731. https://doi.org/10.3390/ijms21186731