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Article

Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

1
Unit for Reproductive Medicine of Clinics/Clinic for Pigs and Small Ruminants, University of Veterinary Medicine, 30559 Hannover, Germany
2
Paul Langerhans Institute Dresden, Helmholtz Zentrum München, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany
3
German Centre for Diabetes Research (DZD e.V.), 85764 Neuherberg, Germany
4
Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians-University Munich, 81377 Munich, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(18), 6474; https://doi.org/10.3390/ijms21186474
Received: 22 July 2020 / Revised: 1 September 2020 / Accepted: 2 September 2020 / Published: 4 September 2020
Long-term exposure of liquid preserved boar spermatozoa to seminal plasma (SP) can cause dramatic sperm injury. This study examined whether boar specificity exists in the sensitivity of spermatozoa to SP and whether correspondent biomarkers can be identified. Consecutive ejaculates (n = 4–5) collected from 19 boars were centrifuged, diluted with a pH-stablising extender with 10% (v/v) autologous SP and evaluated by computer-assisted semen analysis and flow cytometry. Up until 144 h storage, four boars showed consistently high sperm motility, viability and mitochondria activity, and one boar showed consistently low values. Intra-boar variability was high in the other boars. Screening of SP (n = 12 samples) for protein markers using mass spectrometry identified three protein candidates of which the granulin precursor, legumain and AWN were 0.5 to 0.9 log2-fold less abundant (p < 0.05) in SP-resistant compared to SP-sensitive samples. Lipidome analysis by mass spectrometry revealed 568 lipids showing no difference between the SP-groups. The most abundant lipids were cholesterol (42,442 pmol), followed by phosphatidylserine (20,956 pmol) and ether-linked phosphatidylethanolamine (13,039 pmol). In conclusion, three candidate proteins were identified which might be indicative of SP-tolerance of sperm during long-term storage. Noteworthy, a first lipidomic profile of boar SP is presented. View Full-Text
Keywords: boar seminal plasma; sperm quality; semen preservation; biomarker; proteomics; lipidomics boar seminal plasma; sperm quality; semen preservation; biomarker; proteomics; lipidomics
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MDPI and ACS Style

Höfner, L.; Luther, A.-M.; Palladini, A.; Fröhlich, T.; Waberski, D. Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach. Int. J. Mol. Sci. 2020, 21, 6474. https://doi.org/10.3390/ijms21186474

AMA Style

Höfner L, Luther A-M, Palladini A, Fröhlich T, Waberski D. Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach. International Journal of Molecular Sciences. 2020; 21(18):6474. https://doi.org/10.3390/ijms21186474

Chicago/Turabian Style

Höfner, Lisa, Anne-Marie Luther, Alessandra Palladini, Thomas Fröhlich, and Dagmar Waberski. 2020. "Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach" International Journal of Molecular Sciences 21, no. 18: 6474. https://doi.org/10.3390/ijms21186474

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