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Lard Injection Matrix for Serial Crystallography

Department of Life Science, Pohang University of Science and Technology, Pohang 37673, Korea
Int. J. Mol. Sci. 2020, 21(17), 5977;
Received: 4 August 2020 / Revised: 17 August 2020 / Accepted: 18 August 2020 / Published: 19 August 2020
(This article belongs to the Special Issue Molecular Dynamics from Macromolecule to Small Molecules)
Serial crystallography (SX) using X-ray free electron laser or synchrotron X-ray allows for the determination of structures, at room temperature, with reduced radiation damage. Moreover, it allows for the study of structural dynamics of macromolecules using a time-resolved pump-probe, as well as mix-and-inject experiments. Delivering a crystal sample using a viscous medium decreases sample consumption by lowering the flow rate while being extruded from the injector or syringe as compared to a liquid jet injector. Since the environment of crystal samples varies, continuous development of the delivery medium is important for extended SX applications. Herein, I report the preparation and characterization of a lard-based sample delivery medium for SX. This material was obtained using heat treatment, and then the soluble impurities were removed through phase separation. The lard injection medium was highly stable and could be injected via a syringe needle extruded at room temperature with a flow rate < 200 nL/min. Serial millisecond crystallography experiments were performed using lard, and the room temperature structures of lysozyme and glucose isomerase embedded in lard at 1.75 and 1.80 Å, respectively, were determined. The lard medium showed X-ray background scattering similar or relatively lower than shortenings and lipidic cubic phase; therefore, it can be used as sample delivery medium in SX experiments. View Full-Text
Keywords: serial crystallography; serial millisecond crystallography; sample delivery; viscous medium; lard; fat serial crystallography; serial millisecond crystallography; sample delivery; viscous medium; lard; fat
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MDPI and ACS Style

Nam, K.H. Lard Injection Matrix for Serial Crystallography. Int. J. Mol. Sci. 2020, 21, 5977.

AMA Style

Nam KH. Lard Injection Matrix for Serial Crystallography. International Journal of Molecular Sciences. 2020; 21(17):5977.

Chicago/Turabian Style

Nam, Ki H. 2020. "Lard Injection Matrix for Serial Crystallography" International Journal of Molecular Sciences 21, no. 17: 5977.

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