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Open AccessArticle

Collagen 24 α1 Is Increased in Insulin-Resistant Skeletal Muscle and Adipose Tissue

1
Division of Systems Medicine, School of Medicine, University of Dundee, Dundee, Scotland DD1 9SY, UK
2
Drug Discovery Unit, University of Dundee, Dundee, Scotland DD1 9SY, UK
3
Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, Scotland EH16 4TJ, UK
4
Department of Molecular Physiology and Biophysics and Mouse Metabolic Phenotyping Centre, Vanderbilt University, Nashville, TN 37232, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(16), 5738; https://doi.org/10.3390/ijms21165738
Received: 29 June 2020 / Revised: 29 July 2020 / Accepted: 2 August 2020 / Published: 10 August 2020
Aberrant extracellular matrix (ECM) remodelling in muscle, liver and adipose tissue is a key characteristic of obesity and insulin resistance. Despite its emerging importance, the effective ECM targets remain largely undefined due to limitations of current approaches. Here, we developed a novel ECM-specific mass spectrometry-based proteomics technique to characterise the global view of the ECM changes in the skeletal muscle and liver of mice after high fat (HF) diet feeding. We identified distinct signatures of HF-induced protein changes between skeletal muscle and liver where the ECM remodelling was more prominent in the muscle than liver. In particular, most muscle collagen isoforms were increased by HF diet feeding whereas the liver collagens were differentially but moderately affected highlighting a different role of the ECM remodelling in different tissues of obesity. Moreover, we identified a novel association between collagen 24α1 and insulin resistance in the skeletal muscle. Using quantitative gene expression analysis, we extended this association to the white adipose tissue. Importantly, collagen 24α1 mRNA was increased in the visceral adipose tissue, but not the subcutaneous adipose tissue of obese diabetic subjects compared to lean controls, implying a potential pathogenic role of collagen 24α1 in obesity and type 2 diabetes. View Full-Text
Keywords: extracellular matrix; collagen; obesity; diabetes extracellular matrix; collagen; obesity; diabetes
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Weng, X.; Lin, D.; Huang, J.T.J.; Stimson, R.H.; Wasserman, D.H.; Kang, L. Collagen 24 α1 Is Increased in Insulin-Resistant Skeletal Muscle and Adipose Tissue. Int. J. Mol. Sci. 2020, 21, 5738.

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