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Article

An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols

1
BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, Switzerland
2
Institute of Experimental Medicine of the Czech Academy of Sciences, 142 20 Prague, Czech Republic
3
Department of Chemistry, University of Fribourg, 1700 Fribourg, Switzerland
4
Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh EH14 4AS, UK
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(15), 5335; https://doi.org/10.3390/ijms21155335
Received: 12 June 2020 / Revised: 14 July 2020 / Accepted: 22 July 2020 / Published: 27 July 2020
(This article belongs to the Special Issue Interaction of Nanomaterials with the Immune System)
In vitro three-dimensional (3D) lung cell models have been thoroughly investigated in recent years and provide a reliable tool to assess the hazard associated with nanomaterials (NMs) released into the air. In this study, a 3D lung co-culture model was optimized to assess the hazard potential of multiwalled carbon nanotubes (MWCNTs), which is known to provoke inflammation and fibrosis, critical adverse outcomes linked to acute and prolonged NM exposure. The lung co-cultures were exposed to MWCNTs at the air-liquid interface (ALI) using the VITROCELL® Cloud system while considering realistic occupational exposure doses. The co-culture model was composed of three human cell lines: alveolar epithelial cells (A549), fibroblasts (MRC-5), and macrophages (differentiated THP-1). The model was exposed to two types of MWCNTs (Mitsui-7 and Nanocyl) at different concentrations (2–10 μg/cm2) to assess the proinflammatory as well as the profibrotic responses after acute (24 h, one exposure) and prolonged (96 h, repeated exposures) exposure cycles. The results showed that acute or prolonged exposure to different concentrations of the tested MWCNTs did not induce cytotoxicity or apparent profibrotic response; however, suggested the onset of proinflammatory response. View Full-Text
Keywords: lung; in vitro; co-culture; carbon nanotubes; multiwalled carbon nanotubes; air-liquid interface; toxicity; proinflammatory; profibrotic lung; in vitro; co-culture; carbon nanotubes; multiwalled carbon nanotubes; air-liquid interface; toxicity; proinflammatory; profibrotic
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MDPI and ACS Style

Barosova, H.; Karakocak, B.B.; Septiadi, D.; Petri-Fink, A.; Stone, V.; Rothen-Rutishauser, B. An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols. Int. J. Mol. Sci. 2020, 21, 5335. https://doi.org/10.3390/ijms21155335

AMA Style

Barosova H, Karakocak BB, Septiadi D, Petri-Fink A, Stone V, Rothen-Rutishauser B. An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols. International Journal of Molecular Sciences. 2020; 21(15):5335. https://doi.org/10.3390/ijms21155335

Chicago/Turabian Style

Barosova, Hana, Bedia B. Karakocak, Dedy Septiadi, Alke Petri-Fink, Vicki Stone, and Barbara Rothen-Rutishauser. 2020. "An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols" International Journal of Molecular Sciences 21, no. 15: 5335. https://doi.org/10.3390/ijms21155335

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