Next Article in Journal
B-Cell-Activating Factor Depletion Ameliorates Aging-Dependent Insulin Resistance via Enhancement of Thermogenesis in Adipose Tissues
Next Article in Special Issue
Systematic Review—The Potential Implications of Different Platelet-Rich Plasma (PRP) Concentrations in Regenerative Medicine for Tissue Repair
Previous Article in Journal
Pro-Fibrotic Phenotype in a Patient with Segmental Stiff Skin Syndrome via TGF-β Signaling Overactivation
Previous Article in Special Issue
Regulation of Human Platelet Activation and Prevention of Arterial Thrombosis in Mice by Auraptene through Inhibition of NF-κB Pathway
 
 
Article
Peer-Review Record

Platelet Lysate Induces in Human Osteoblasts Resumption of Cell Proliferation and Activation of Pathways Relevant for Revascularization and Regeneration of Damaged Bone

Int. J. Mol. Sci. 2020, 21(14), 5123; https://doi.org/10.3390/ijms21145123
by Van Thi Nguyen 1,†, Marta Nardini 2,3,†, Alessandra Ruggiu 1, Ranieri Cancedda 4, Fiorella Descalzi 1 and Maddalena Mastrogiacomo 2,3,5,*
Reviewer 1: Anonymous
Reviewer 2:
Int. J. Mol. Sci. 2020, 21(14), 5123; https://doi.org/10.3390/ijms21145123
Submission received: 25 June 2020 / Revised: 13 July 2020 / Accepted: 16 July 2020 / Published: 20 July 2020
(This article belongs to the Special Issue Molecular Research on Platelet Activity in Health and Disease 2.0)

Round 1

Reviewer 1 Report

The study be Nguyen investigates the effect of platelet-derived molecules on human primary osteoblast functions, including proliferation, differentiation and angiogenesis-related pathways. Osteoblasts were isolated from bone chips from elderly donors (41-85 years of age) undergoing hip joint replacement surgery.

1.The age range of the donors is rather large. How many different donors were used for this study? Were different donor cell populations mixed for one experiment? What was the variation between donors? The authors should include a table indicated which donor was used for which experiment.

2.The in vivo differentiation study was carried out with platelet lysate stimulation, what was the timeframe? What are the different colours in the histological analysis in figure 3? The differences between the histology images are not clear, could the authors perform a quantitative image analysis? How many animals per group were used? Correct spelling mistakes, for example 1.5x106 cells and 2.5x106 cells.

3.The quantification for the 32 hrs timepoint in figure 4b is missing.

4.The authors should include the entire Western blot images for figures 4, 5 and 7 as supplementary items.

5.The quantification for the 32 hrs timepoint in figure 5c is missing.

6.Angiogenesis or bone healing assays should be carried out to confirm the involvement of platelet-derived molecules in osteoblast/endothelial cell interactions.

Author Response

Dear Reviewer,

we addressed your comments into the text hoping to have improved the quality of our manuscript.

Please see the attachment

Best regards

 

M. Mastrogiacomo

Author Response File: Author Response.zip

Reviewer 2 Report

This is a well written paper that demonstrated the effect of PL in osteoblast growth and related angiogenic events. Related signaling pathways are well delineated with detailed molecular studies. Several minor comments are as follow.

 

Line 49-51 : please re-write for clarification

Line 69-74 : please split the sentence and re-write for better understanding

Line 81 : please split the sentence

Line 116 : how can one be sure these cells are osteoblasts ? Tests on specific marker binding seems required.

Line 151 : what is the concentration of PL in the medium ?

Line 267 – 270 : please re-write

Line 336 : Figure 6B : no explanation can be found in the text. What is the intention of showing the stained cells ?

Line 395 – 396 : the thought of this sentence is very unclear

 

Author Response

Dear Reviewer,

we addressed your comments into the text hoping to have improved the quality of our manuscript.

Please see the attachment

Best regards

 

M. Mastrogiacomo

Author Response File: Author Response.zip

Round 2

Reviewer 1 Report

The authors have addressed the comments made. The manuscript text requires minor spell checking (e.g. line 162: 1,5x106; line 167: 3,7%; line 290: 1,5x106 and 2,5x106). The number of animals per group should be included in the methods section 2.5.2.

Back to TopTop