Biodistribution PET/CT Study of Hemoglobin-DFO-89Zr Complex in Healthy and Lung Tumor-Bearing Mice
Abstract
:1. Introduction
2. Results
2.1. Formation of the Human Hb-DFO Conjugate Is Selective, Fast and Quantitative
2.2. DFO Slightly Affects the Orientation of Two Side Chains
2.3. 89Zr Uptake by Hb-DFO Is Fast and Efficient
2.4. Dose-Dependent Biodistribution and Elimination of Hb-DFO-89Zr in Mice: No Preferential Internalization Observed by Damaged Tissues
2.5. Hb Is Not Scavenged by the Liver Macrophages in a Mouse Model
3. Discussion
4. Materials and Methods
4.1. Synthesis of Hb-DFO
4.2. MALDI-TOF Analysis of Hb-DFO
4.3. Crystallization of Hb-DFO
4.4. X-ray Data Collection, Structure Determination and Refinement
4.5. Radiosynthesis of 89Zr Oxalate
4.6. Radiolabelling of Hb-DFO
4.7. Radio-TLC
4.8. Radio-HPLC
4.9. Transplantation of 4T1 Cells to BALB/c Mice
4.10. Hb-DFO-89Zr PET/CT Imaging of BALB/c Mice
4.11. In-Vivo MS FX PRO Imaging and Flow Cytometry
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
Hb | Hemoglobin |
Hp | Haptoglobin |
PET/CT | positron emission tomography/computed tomography |
DFO | deferoxamine |
MALDI-TOF | matrix-assisted laser desorption/ionization-time of flight |
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Kiraga, Ł.; Cerutti, G.; Braniewska, A.; Strzemecki, D.; Sas, Z.; Boffi, A.; Savino, C.; Montemiglio, L.C.; Turnham, D.; Seaton, G.; et al. Biodistribution PET/CT Study of Hemoglobin-DFO-89Zr Complex in Healthy and Lung Tumor-Bearing Mice. Int. J. Mol. Sci. 2020, 21, 4991. https://doi.org/10.3390/ijms21144991
Kiraga Ł, Cerutti G, Braniewska A, Strzemecki D, Sas Z, Boffi A, Savino C, Montemiglio LC, Turnham D, Seaton G, et al. Biodistribution PET/CT Study of Hemoglobin-DFO-89Zr Complex in Healthy and Lung Tumor-Bearing Mice. International Journal of Molecular Sciences. 2020; 21(14):4991. https://doi.org/10.3390/ijms21144991
Chicago/Turabian StyleKiraga, Łukasz, Gabriele Cerutti, Agata Braniewska, Damian Strzemecki, Zuzanna Sas, Alberto Boffi, Carmelinda Savino, Linda Celeste Montemiglio, Daniel Turnham, Gillian Seaton, and et al. 2020. "Biodistribution PET/CT Study of Hemoglobin-DFO-89Zr Complex in Healthy and Lung Tumor-Bearing Mice" International Journal of Molecular Sciences 21, no. 14: 4991. https://doi.org/10.3390/ijms21144991