Chaperone-Like Activity of HSPB5: The Effects of Quaternary Structure Dynamics and Crowding
Abstract
:1. Introduction
2. Results
2.1. Protein Aggregation. Theoretical Analysis of the Initial Parts of the Kinetic Curves
2.2. The Effect of HSPB5 on Thermal Aggregation of Phb
2.3. The Effect of HSPB5 on DTT-Induced Aggregation of α-Lactalbumin (αLa)
2.4. Sedimentation Velocity (SV) Analysis of HSPB5 and α-Lactalbumin at 37 °C
2.5. Sedimentation Velocity Analysis of HSPB5 and Phb at 48 °C
2.6. Sedimentation Velocity Analysis of HSPB5 and Phb at 48 °C Under Crowded Conditions
2.7. Effect of Crowding on the Chaperone-Like Activity of HSPB5
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Dynamic Light Scattering (DLS)
4.3. Analytical Ultracentrifugation (AUC)
4.4. Calculations
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ACD | α-Crystalline domain |
ATP | Adenosine triphosphate |
AUC | Analytical ultracentrifugation |
DLS | Dynamic light scattering |
DTT | Dithiothreitol |
EDTA | Ethylenediaminetetraacetic acid |
EVE | Excluded volume effect |
IDR | Intrinsically disordered region |
PEG | Polyethylene glycol |
PVP | Polyvinylpyrrolidone |
sHSPs | Small heat shock proteins |
SV | Sedimentation velocity |
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[HSPB5] (mg/mL) | s20,w (S) | Friction Ratio, f/f0 | Molecular Mass (kDa) |
---|---|---|---|
0 | 2.9 ± 0.8 6.1 ± 0.4 | 2.7 | |
0.00045 | 1.85 ± 0.13 | 1.614 | 25.4 |
0.0015 | 2.0 ± 0.1 | 1.545 | 26 |
0.0075 | 1.9 ± 0.2 2.4 ± 0.3 | 1.925 | 33 47 |
0.03 | 1.6 ± 0.2 2.0 ± 0.1 | 1.836 | 23.9 37.4 |
Additions | Kagg ((counts/s)/s2) | t0 (s) | 1 | j |
---|---|---|---|---|
Without addition of crowding agents | ||||
– | 0.123 ± 0.007 | 323 ± 9 | 1.0 | - |
Action of individual crowding agents | ||||
PEG20kDa 25 mg/mL | 1.85 ± 0.04 | 287 ± 2 | 15.0 ± 0.9 | - |
PVP10kDa 25 mg/mL | 0.28 ± 0.01 | 266 ± 5 | 2.3 ± 0.2 | - |
PVP25kDa 25 mg/mL | 0.52 ± 0.02 | 232 ± 5 | 4.2 ± 0.3 | - |
Ficoll70kDa 75 mg/mL | 0.144 ± 0.015 | 252 ± 12 | 1.17 ± 0.14 | - |
Combined action of crowding agents | ||||
PEG20kDa 25 mg/mL + Ficoll70kDa 75 mg/mL | 1.37 ± 0.05 | 196 ± 4 | 11.1 ± 0.8 | 0.71 ± 0.06 |
PVP10kDa 25 mg/mL + Ficoll70kDa 75 mg/mL | 1.69 ± 0.05 | 263 ± 3 | 13.7 ± 0.9 | 8.8 ± 0.6 |
PVP25kDa 25 mg/mL + Ficoll70kDa 75 mg/mL | 3.76 ± 0.21 | 240 ± 3 | 30.6 ± 2.4 | 8.7 ± 0.7 |
PVP10kDa 25 mg/mL + PEG20kDa 25 mg/mL | 4.00 ± 0.22 | 227 ± 3 | 32.5 ± 2.6 | 2.06 ± 0.17 |
PVP25kDa 25 mg/mL + PEG20kDa 25 mg/mL | 6.76 ± 0.29 | 203 ± 2 | 55 ± 4 | 3.12 ± 0.23 |
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Chebotareva, N.A.; Roman, S.G.; Borzova, V.A.; Eronina, T.B.; Mikhaylova, V.V.; Kurganov, B.I. Chaperone-Like Activity of HSPB5: The Effects of Quaternary Structure Dynamics and Crowding. Int. J. Mol. Sci. 2020, 21, 4940. https://doi.org/10.3390/ijms21144940
Chebotareva NA, Roman SG, Borzova VA, Eronina TB, Mikhaylova VV, Kurganov BI. Chaperone-Like Activity of HSPB5: The Effects of Quaternary Structure Dynamics and Crowding. International Journal of Molecular Sciences. 2020; 21(14):4940. https://doi.org/10.3390/ijms21144940
Chicago/Turabian StyleChebotareva, Natalia A., Svetlana G. Roman, Vera A. Borzova, Tatiana B. Eronina, Valeriya V. Mikhaylova, and Boris I. Kurganov. 2020. "Chaperone-Like Activity of HSPB5: The Effects of Quaternary Structure Dynamics and Crowding" International Journal of Molecular Sciences 21, no. 14: 4940. https://doi.org/10.3390/ijms21144940