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Article

A Possible Role for Arylsulfatase G in Dermatan Sulfate Metabolism

1
Department of Systems Biology and Engineering, Silesian University of Technology, 44-100 Gliwice, Poland
2
Department of Cardiology, 4th Municipal Hospital, 44-100 Gliwice, Poland
3
Department of Pediatrics, Nutrition and Metabolic Diseases, The Children’s Memorial Health Institute, 04-730 Warsaw, Poland
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(14), 4913; https://doi.org/10.3390/ijms21144913
Received: 2 June 2020 / Revised: 1 July 2020 / Accepted: 6 July 2020 / Published: 12 July 2020
(This article belongs to the Special Issue Genetic and Metabolic Molecular Research of Lysosomal Storage Disease)
Perturbations of glycosaminoglycan metabolism lead to mucopolysaccharidoses (MPS)—lysosomal storage diseases. One type of MPS (type VI) is associated with a deficiency of arylsulfatase B (ARSB), for which we previously established a cellular model using pulmonary artery endothelial cells with a silenced ARSB gene. Here, we explored the effects of silencing the ARSB gene on the growth of human pulmonary artery smooth muscle cells in the presence of different concentrations of dermatan sulfate (DS). The viability of pulmonary artery smooth muscle cells with a silenced ARSB gene was stimulated by the dermatan sulfate. In contrast, the growth of pulmonary artery endothelial cells was not affected. As shown by microarray analysis, the expression of the arylsulfatase G (ARSG) in pulmonary artery smooth muscle cells increased after silencing the arylsulfatase B gene, but the expression of genes encoding other enzymes involved in the degradation of dermatan sulfate did not. The active site of arylsulfatase G closely resembles that of arylsulfatase B, as shown by molecular modeling. Together, these results lead us to propose that arylsulfatase G can take part in DS degradation; therefore, it can affect the functioning of the cells with a silenced arylsulfatase B gene. View Full-Text
Keywords: arylsulfatase; dermatan sulfate; mucopolysaccharidosis; smooth muscle cell arylsulfatase; dermatan sulfate; mucopolysaccharidosis; smooth muscle cell
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MDPI and ACS Style

Poterala-Hejmo, A.; Golda, A.; Pacholczyk, M.; Student, S.; Tylki-Szymańska, A.; Lalik, A. A Possible Role for Arylsulfatase G in Dermatan Sulfate Metabolism. Int. J. Mol. Sci. 2020, 21, 4913. https://doi.org/10.3390/ijms21144913

AMA Style

Poterala-Hejmo A, Golda A, Pacholczyk M, Student S, Tylki-Szymańska A, Lalik A. A Possible Role for Arylsulfatase G in Dermatan Sulfate Metabolism. International Journal of Molecular Sciences. 2020; 21(14):4913. https://doi.org/10.3390/ijms21144913

Chicago/Turabian Style

Poterala-Hejmo, Aleksandra; Golda, Adam; Pacholczyk, Marcin; Student, Sebastian; Tylki-Szymańska, Anna; Lalik, Anna. 2020. "A Possible Role for Arylsulfatase G in Dermatan Sulfate Metabolism" Int. J. Mol. Sci. 21, no. 14: 4913. https://doi.org/10.3390/ijms21144913

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