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Open AccessArticle

Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer

1
Institute of Health Sciences, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzeszów, Poland
2
Medical College of Rzeszow University, Institute of Medical Sciences, Kopisto 2a, 35-959 Rzeszów, Poland
3
Department of Pathomorphology, Chair of Morphological Sciences, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzeszow, Poland
4
Institute of Nuclear Physics, Polish Academy of Science, Radzikowskiego 152, 31-342 Krakow, Poland
5
Institute of Physics, College of Natural Sciences, University of Rzeszow, - St. Pigonia 1, 35-310 Rzeszów, Poland
6
Clinic of Paediatric Oncology and Haematology, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzeszow, Poland
7
Department of Human Histology, Chair of Morphological Sciences, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzeszow, Poland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(14), 4828; https://doi.org/10.3390/ijms21144828
Received: 17 June 2020 / Revised: 26 June 2020 / Accepted: 6 July 2020 / Published: 8 July 2020
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Currently, endometrial carcinoma (EC) is the most common genital cancer in high-income countries. Some types of endometrial hyperplasia (EH) may be progressing to this malignancy. The diagnosis of EC and EH is based on time consuming histopathology evaluation, which is subjective and causes discrepancies in reassessment. Therefore, there is a need to create methods of objective evaluation allowing the diagnosis of early changes. The study aimed to simultaneously asses Fourier Transform Infrared (FTIR) and Raman spectroscopy combined with multidimensional analysis to identify the tissues of endometrial cancer, atypical hyperplasia and the normal control group, and differentiate them. The results of FTIR and Raman spectroscopy revealed quantitative and qualitative changes in the nucleic acid and protein in the groups of cancer and atypical hyperplasia, in comparison with the control group. Changes in the lipid region were also observed in Raman spectra. Pearson correlation coefficient demonstrated a statistically significant correlation between Raman spectra for the cancer and atypical hyperplasia groups (0.747, p < 0.05) and for atypical hyperplasia and the controls (0.507, p < 0.05), while FTIR spectra demonstrated a statistically significant positive correlation for the same group as in Raman data and for the control and cancer groups (0.966, p < 0.05). To summarize, the method of spectroscopy enables differentiation of atypical hyperplasia and endometrial cancer tissues from the physiological endometrial tissue. View Full-Text
Keywords: endometrial cancer; atypical hyperplasia; FTIR spectroscopy; Raman spectroscopy; correlation test endometrial cancer; atypical hyperplasia; FTIR spectroscopy; Raman spectroscopy; correlation test
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Barnas, E.; Skret-Magierlo, J.; Skret, A.; Kaznowska, E.; Depciuch, J.; Szmuc, K.; Łach, K.; Krawczyk-Marć, I.; Cebulski, J. Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer. Int. J. Mol. Sci. 2020, 21, 4828.

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